Redox dye and method of preparation thereof using 2-hydroxypropy

Details Redox dye and method of preparation thereof using 2-hydroxypropy Redox dye and method of preparation thereof using 2-hydroxypropy

1993-07-28

1995-07-11

Knode, Marian C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536124, 556143, 435 41, 435 72, 435101, 435176, 435177, C08B 3716, C07F 1702, C12P 1904, C12N 1114

Patent

active

054322740

ABSTRACT:
Ferricinium or ferricinium-based compounds are useful as blue redox dyes suitable for monitoring various enzymatic oxidation processes. A process for preparing such dyes is proposed which involves complexing ferrocene or its derivative with a cyclodextrin. The resulting yellow inclusion complex, an intermediate of the redox dye, is then oxidized electrochemically or by a reaction with bilirubin oxidase to form blue redox dyes which exhibit absorption peaks at 620 or 650 nm. Both the reduced form and the oxidized form of the dye are water-soluble.

REFERENCES:
patent: 5116730 (1992-05-01), Artiss et al.
patent: 5200181 (1993-04-01), Soltys et al.
Luong et al, Anal. Biochem. 212 (1): 269-276 (1993).
Harada et al, J. Chem. Soc. Dalton Trans., pp. 729-732 (1988).
Strelets et al, J. Electroanal Chem 310:179-186 (1991).
Ryabov et al, Bioelectrochem. Bioenerg. 24(2): 257-262 (1990).
Aldrich Catalogue, pp. 501, 714-715 (1992).
Sigma Catalogue, p. 459, (1992).

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