Red absorbing combination of alcohol oxidase and an azide compou

Details Red absorbing combination of alcohol oxidase and an azide compou Red absorbing combination of alcohol oxidase and an azide compou

1980-11-04

1984-02-07

Nafe, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435188, 435189, 435190, 435814, 435815, 435816, C12Q 126, C12N 902, C12N 904, C12N 996

Patent

active

044304277

ABSTRACT:
Combining active alcohol oxidase with sufficient amount of an azide compound has been found to form a red absorbing combination. Formation of the red absorbing combination enables determining the presence of active alcohol oxidase by adding the azide compound to a preparation and observing the resultant color. Additionally, alcohol oxidase may be purified by adding the azide compound to a preparation containing active alcohol oxidase in an amount effective to produce a red absorbing complex and separating the red absorbing complex from the preparation.

REFERENCES:
patent: 3627640 (1971-12-01), Blumberg et al.
patent: 3985617 (1976-10-01), Yugari et al.
patent: 4160698 (1979-07-01), Miyairi et al.
patent: 4250254 (1981-02-01), Modrovich
patent: 4250261 (1981-02-01), Eggeling et al.
Bringer, et al., Purification and Properties of Alcohol Oxidase from Poria contigua, Eur. J. Biochem. vol. 101, 11/79, (pp. 563-570).

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