Recycle process for the production of low-cost soluble collagen

Details Recycle process for the production of low-cost soluble collagen Recycle process for the production of low-cost soluble collagen

1994-05-27

1995-10-24

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

435 681, 435 711, 435184, 435212, 435213, 435219, 530356, C12P 2100, C12N 999

Patent

active

054609674

ABSTRACT:
The invention includes a method that produces a low cost aqueous solution of solubilized collagen by the steps of: (a) providing an aqueous ground slurry of insoluble collagen and adjusting the pH of said slurry to obtain activity for a proteolytic enzyme added in Step b; (b) adding said proteolytic enzyme to said pH adjusted slurry; (c) reacting the slurry and enzyme of Step b and/or recycled insoluble collagen and enzyme from Step e at a temperature, T, and for a time, t, effective for forming a solution increased in solubilized collagen; (d) adding additional water and insoluble collagen to said solution of Step c and mixing; (e) separating at least some of the solution of Step d containing solubilized collagen from insoluble collagen, whereby at least a portion of said insoluble collagen and proteolytic enzyme is recycled to Step c, and the separated solution containing solubilized collagen is withdrawn as product; an alternative embodiment provides for the direct production of solubilized collagen without the recycle step.

REFERENCES:
patent: 4066083 (1978-01-01), Ries
patent: 4220724 (1980-09-01), Berg et al.
patent: 4389487 (1983-06-01), Ries
patent: 5316942 (1994-05-01), Fink

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