Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1989-12-12
1992-11-10
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 14, 435 723, 435 71, 436501, 436518, 436808, 436 64, 436813, C12Q 126, C12Q 154, G01N 33574
Patent
active
051622025
ABSTRACT:
An improved rectal mucus screening test method and kit for cancerous and precancerous conditions which is rapid, employs reagents which can be provided in kit form and which does not give false negatives due to sampling error, immobilizes the mucus sample in a membrane filter, tests for marker carbohydrates which have vicinal galactose moieties and which are oxidized with galactose oxidase to vicinal aldehydic moieties which are visualized with Schiff's reagent and oxidizes those samples which test negative for the marker carbohydrates with periodic acid, renders the sample visualizable with Schiff's reagent, applies the galactose oxidase directly to the membrane filter, thereby speeding up color developed and employs the Schiff's Reagent which was refrigerated after preparation until the color faded to a straw shade before being treated with activated charcoal, which renders the solution storage stable ability for many months and enhances its color development ability.
REFERENCES:
patent: 4857457 (1989-08-01), Shamasuddin et al.
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Fairbanks et al., "Electrophoretic Analysis of the Major Polypeptides of the Human Erythrocyte Membrane" Biochemistry vol. 10, No. 13 1971 pp. 2606-2617.
Dutt, M. K., Biological Abstracts, vol. 73, Abstract No. 69469 (1981).
Dutt; M. K., Biological Abstracts, vol. 72, Abstract No. 74604 (1981).
Dutt; M. K., Biological Abstracts, vol. 63, Abstract No. 16544 (1976).
Sakamoto et al., "A Pilot Study on the Usefulness of a New Test for Mass Screening of Colorectal Cancer in Japan," Gastroenterologia Japonica, vol. 25, No. 4, pp. 432-436 (1990).
McKenzie et al., "Expression of Carcinoembryonic Antigen, T-Antigen, and Oncogene Products as Markers of Neoplastic and Preneoplastic Colonic Mucosa," Human Pathology, vol. 18, No. 12, pp. 1282-1286 (Dec. 1987).
Kepplinger Esther L.
Scheiner Toni R.
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