Recovery of urokinase compounds

Details Recovery of urokinase compounds Recovery of urokinase compounds

1988-06-10

1990-04-24

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435815, 424 9463, C12N 972

Patent

active

049200518

ABSTRACT:
Disclosed is a method of recovering urokinase compounds from media and cell extracts. The method comprises contacting the urokinase compound-containing solution with a silicaceous matrix material comprising covalently bound polymer having plural anionic groups. Selective elution of the urokinase compound can produce elutants of high specific activity. The method can succeed in recovering greater than 90% of the urokinase activity from the crude solution.

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