Recovery of tissue plasminogen activator

Details Recovery of tissue plasminogen activator Recovery of tissue plasminogen activator

1988-02-03

1990-05-29

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435219, 435815, C12N 964, C12N 948, C12N 950

Patent

active

049295608

ABSTRACT:
Disclosed is a method of recovering tissue plasminogen activator from media and cell extracts. The method comprises contacting the tPA-containing solution with a silicaceous matrix material comprising covalently bound polymer having plural anionic groups. Selective elution of the tPA can produce eluants of high specific activity. The method can succeed in recovering greater than 90% of the tPA activity from the crude solution.

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Nau (1986) "A Unique Chromatographic Matrix for Rapid Antibody Purification" (Research Specialty Products Division, J. T. Baker Chemical Co.) BioChromatography, vol. 1, No. 2, pp. 82-94.

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