Recovery of oligonucleotides by gas phase cleavage

Details Recovery of oligonucleotides by gas phase cleavage Recovery of oligonucleotides by gas phase cleavage

1996-02-15

1998-04-14

Bhat, Nina

Chemical apparatus and process disinfecting, deodorizing, preser

Control element responsive to a sensed operating condition

Control element directly mechanically linked to separate sensor

422131, 422134, 4353052, G05D 2300, C08F 200

Patent

active

057388296

ABSTRACT:
A process and apparatus for the cleavage and deprotection of newly synthesized oligonucleotides from solid supports, which involves incubating the solid support in an environment comprising gaseous cleavage/deprotection reagent such as gaseous ammonia or ammonium hydroxide vapors. The method lends itself well to the use of supports such as microtiter plates, that can be used to simultaneously perform up to 96 individual synthetic processes.

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patent: 5393877 (1995-02-01), McLean et al.
patent: 5460786 (1995-10-01), Nokihara
Andrew M. Bray, et al., "Gas Phase Cleavage of Peptides from a Solid Support with Ammonia Vapour. Application in Simultaneous Multiple Peptide Synthesis,"Tetrahedron Letters, vol. 32, No. 43, pp. 6163-6166 (1991).
"Leroy Hood: Thinking Big in Seattle", Science 264:206-209 (1994).

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