Recovery of linear nucleic acids by salt dilution and/or...

Details Recovery of linear nucleic acids by salt dilution and/or... Recovery of linear nucleic acids by salt dilution and/or...

2006-07-11

2006-07-11

Kim, John (Department: 1723)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S639000, C210S644000, C210S651000, C210S767000, C435S006120, C435S091200, C536S023100, C536S025400

Reexamination Certificate

active

07074333

ABSTRACT:
A method of recovery of DNA fragments using ultrafiltration. The method of the present invention relates to the separation and/or fractionation of double stranded linear nucleic acids using continuous pressure differential ultrafiltration to achieve improved recovery of small PCR products. In one embodiment, dilution of sample such as small PCR products is carried out prior to purification by ultrafiltration. Subsequent filtration of linear DNA, for example, under diluted conditions significantly increases the recovery of smaller PCR products, and improves the recovery of larger DNA fragments as well. In another embodiment, reduced vacuum pressure is used during ultrafiltration to increase the recovery of smaller PCR products and improve the recovery of larger DNA fragments as well.

REFERENCES:
patent: 4632761 (1986-12-01), Bowers et al.
patent: 4772972 (1988-09-01), Maeda
patent: 4832851 (1989-05-01), Bowers et al.
patent: 5141718 (1992-08-01), Clark
patent: 5223133 (1993-06-01), Clark et al.
patent: 5434048 (1995-07-01), Simon et al.
patent: 5543305 (1996-08-01), Cummins et al.
patent: 5589342 (1996-12-01), Geiger et al.
patent: 5596092 (1997-01-01), Schneider
patent: 5707812 (1998-01-01), Horn et al.
patent: 5856100 (1999-01-01), Hayashizaki
patent: 5876936 (1999-03-01), Ju
patent: 0 442 026 (1991-08-01), None
patent: 00/66723 (2000-11-01), None
patent: 01/19482 (2001-03-01), None
patent: 01/79486 (2001-10-01), None
Di James, “Sequencinb Principles and Methods”, University of Cape Town, Mar. 2001.□□Printed from the internet on Dec. 1, 2005: << http://www.mcb.uct.ac.za/principles%20of%20DNA%20sequencing/sld001.htm >>.
Anal. Chem. 1998, 70, 1516-1527; Ruiz-Martinez, et al.; “A Sample Purification Meth d for Rugged and high-performance DNA Sequencing by Capillary Electrophoresis Using Replaceable Polymer Solutions. A. Development of the Cleanup Protocol”.
Anal. Chem. 1998, 70, 1628-1535; Salas-Solano, et al.; “A Sample Purification Method for Rugged and High-Performance DNA Sequencing by Capillary Electrophoresis Using Replaceable Polymer Solutions. B. Quantitative Determination of the Role of Sample Matrix Components on Sequencing Analysis”.
BioTechniques vol. 19, No. 2 (1995); pp. 176-177; Benchmarks; “Method for Sequencing Foreign Genes Expressed from the Polyhedrin Promoter of Recombinant Baculoviruses”.
MEMBRANE, 25 (4), 206-210 (2000); Chaudry, et al.; “High Throughput Sample Preparation using Ultrafiltration in Multiwell Plate Format: Post-Sequencing Cleanup f Dye Terminator Sequencing Reactions for Capillary Electrophoresis”.
Applied and Theoretical Electrophoresis (1996), 6, 11-14; Devaney et al.; “The evaluation of fast purification methods for preparing polymerase chain reaction (PCR) products for capillary electrophoresis analysis”.
BioTechniques, vol. 26, No. 5 (1999); Fangan, et al.; Product Application Focus; “Automated System for Purification of Dye-Terminator Sequencing Products Eliminates Up-Stream Purification of Templates”.
Journal of Chromatograph B, 683 (1996) 109-114; Belgrader, et al.; “Automated polymerase chain reaction product sample preparation for capillary electrophoresis analysis”.
Rapid Communicati ns in Mass Spectrometry, vol. 10, 1475-1478 (1996); M uradian, et al.; “Analyzing Sequencing Reactions from Bacteriophage M13 Matrix-assisted Laser Description/Ionization Mass Spectrometry”.
WPI abstract Acc. No. 1995-113432[15] of RU 2017496 C (Vajnberg) Aug. 15, 1994.
Copy of the Search Report dated Jul. 25, 2002.
MultiScreen-384-PCR Plates User Guide P36239 Nr.Apr. 2000 von 2000.
Reek, F.H. u.a.: Use of Multiscreen plates for the preparation of bacterial DNA suitable for PCR Biotechniques (1995) 19 (2) 282-5.
QIAquick Multiwell PCR Purification Handbook May 1999.
Copy of the foreign communication dated Oct. 2004 with English translation.

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