Recovery of functional human leukocytes from recycled filters

Details Recovery of functional human leukocytes from recycled filters Recovery of functional human leukocytes from recycled filters

1997-12-22

1999-11-23

Walker, W. L.

Liquid purification or separation

Processes

Separating

210798, 210411, 435 2, B01D 2446

Patent

active

059894415

ABSTRACT:
A method of recovering functional human leukocytes from blood filters used to deplete leukocyte content from leukocyte-containing blood-cell suspensions is provided. More particularly, this method involves isolating leukocytes from filters which have been used to purify red blood cells or platelets in isolation from leukocytes by back-flushing the used filters with a hemolysis solution, such as cold ammonium chloride, and collecting the functional human leukocytes.

REFERENCES:
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patent: 4833077 (1989-05-01), Abe et al.
patent: 5833866 (1998-11-01), Brown
A. Abbas et al, "Introduction to Immunology", Cellular and Molecular Immunology, p. 14, W. B. Saunders Company (1991).
R. Longley et al, "Recovery of Functional Human Lymphocytes from Leukotrap Filters", J. Immunol. Meth., 121:33-38 (1989).
J. Coligan, "Preparation of Human Mononuclear Cell Populations and Subpopulations", Current Protocols in Immunology, Section I, Units 7.1-7.2, John Wiley & Sons (1994).
I. Menke-Mollars et al, "Leukocyte Depletion, a Comparison of the `Leukotrap in-line` Filter System with a Sterile Added 4-bag Speacell-R-500-A Filter System", Bietr Infusionsther, 30:152-156 91992) (Abstract).

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