Recovery of enzymes with ion exchangers

Details Recovery of enzymes with ion exchangers Recovery of enzymes with ion exchangers

1975-01-16

1976-07-13

Shapiro, Lionel M.

Food or edible material: processes, compositions, and products

Processes

Separating a starting material into plural different...

195 31R, 195 31F, 195 66R, C07g 702, A23c 914

Patent

active

039695387

ABSTRACT:
A process for separating enzymes from an aqueous solution in which they are mixed with smaller organic molecules, comprising passing the solution through an ion exchange resin, eluting the resin with water, and separating an enzyme-rich fraction before the appearance of a fraction of the eluate containing the smaller organic molecules. The solution and resin are preferably adjusted to that pH from 3 to 9 which is optimum for the catalytic action of the enzyme so the enzyme-rich fraction can thereafter directly be used for biocatalysis. The process can be used to separate lactose from milk, to separate hydrolases from the product obtained by treatment of polysaccharides, invertases from the product obtained by treatment of low saccharides, as well as of hydrolases and invertases jointly from the product obtained by treatment of polysaccharides.

REFERENCES:
patent: 3642580 (1972-02-01), Ghose
patent: 3649456 (1972-03-01), Benneville et al.
patent: 3720583 (1973-03-01), Fisher
methods in Enzymology, vol. 22, pp. 287-321.

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