Recovery of BT endotoxin protein from lysed cell mixtures

Details Recovery of BT endotoxin protein from lysed cell mixtures Recovery of BT endotoxin protein from lysed cell mixtures

1989-12-08

1992-12-22

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 712, 435 713, 43525231, 4352525, 435803, 435832, C12P 2104, C12R 107

Patent

active

051734095

ABSTRACT:
Purified endotoxin protein is recovered from aqueous suspensions containing lysed cells and Bacillus thuringiensis crystalline endotoxin protein in a multistep process.
The aqueous suspension, e.g., a concentrated fermentation culture, is first treated under strongly basic or acidic conditions to solubilize the crystal protein and the solution is then separated from residual solids. Endotoxin protein is precipitated from the separated aqueous solution, by adjusting its pH to the isoelectric point of the protein, and recovered by centrifugation or the like.

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