Recovery of and uses of specific binding agents

Details Recovery of and uses of specific binding agents Recovery of and uses of specific binding agents

1997-10-22

1999-11-23

Housel, James C.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

435 71, 435 795, 435961, 436507, 436510, 436512, 436518, 436541, 436547, 436 8, 436 16, 5303892, G01N 33533

Patent

active

059899263

ABSTRACT:
An antibody specific for a target analyte is purified by affinity chromatography on a substrate bearing a low-affinity analogue of the target analyte. The antibody is displaced from the substrate by contact with a second analogue of intermediate affinity, which remains complexed with the antibody. This complex can be used in a conventional assay for the target analyte, which displaces the intermediate-affinity analogue. The complexed antibody is rendered more storage-stable because the second analogue protects the antibody binding reagion.

REFERENCES:
patent: 4952569 (1990-08-01), Simons
patent: 5467778 (1995-11-01), Catt et al.
patent: 5527686 (1996-06-01), Fitzpatrick et al.
Voet et al. Biochemistry, pp. 90-92, 1990.

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