Recovery of acid fungal protease

Details Recovery of acid fungal protease Recovery of acid fungal protease

1983-12-08

1985-07-30

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435223, 435205, 435814, C12N 934, C12N 958, C12N 962

Patent

active

045322139

ABSTRACT:
Glucoamylase and acid fungal protease co-produced by the fermentation of fungal species capable of producing these enzymes are separated by carrying out the fermentation at a pH at which the protease associates itself with the fungal mycelium and removing the mycelium from the fermentation broth. The mycelium is then resuspended in an aqueous medium and the pH adjusted to cause the dissociation of the protease and mycelium with subsequent recovery of the protease from the aqueous medium.

REFERENCES:
patent: 3892629 (1975-07-01), Smith et al.
Tomonaga, J. Gen. Appl. Microbiol., 12, 267, (1966).
Tomonaga et al., J. Gen. Appl. Microbiol., 10, 373 (1964).
Ichishima et al., Biochem. Biophys. Acta, 99, 360, (1965).

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