Reconstruction and motion analysis of an embryo

Image analysis – Applications – Biomedical applications

Reexamination Certificate

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C382S257000

Reexamination Certificate

active

10409838

ABSTRACT:
In an embodiment, a method includes receiving a number of optical sections of different parts of an embryo over time along a Z-axis through differential interference contrast microscopy. The method also includes outlining a perimeter of the number of parts of the embryo within the number of optical sections. Additionally, the method includes generating a three-dimensional faceted image of the number of parts based on the outlined perimeter. The method includes reconstructing, separately, a four-dimensional image of a growth of the different parts of the embryo over time based on the three-dimensional facet image of the number of parts.

REFERENCES:
patent: 5396418 (1995-03-01), Heuscher
patent: 5655028 (1997-08-01), Soll et al.
patent: 2003/0108231 (2003-06-01), Hamahashi et al.
patent: WO-99/67739 (1999-12-01), None
patent: WO-00/48509 (2000-08-01), None
patent: WO-02/01251 (2002-01-01), None
Soll, “Computer-assisted three-dimensional reconstruction and motion analysis of living, crawling cells,”Computerized Medical Imaging and Graphics, vol. 23, No. 1, Jan. 1999, pp. 3-14.
Heid et al., “3D-DIASemb: A Computer-Assisted System for Reconstructing and Motion Analyzing in 4D Every Cell and Nucleus in a Developing Embryo,”Developmental Biology, vol. 245, No. 2, May 2002, pp. 329-347.
Devries, J. H., et al., “Four-Dimensional Imaging: Computer Visualization of 3D Movements in Living Specimens”,Science, 273, (1996), 603-607.

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