Plastic and nonmetallic article shaping or treating: processes – Encapsulating normally liquid material – Liquid encapsulation utilizing an emulsion or dispersion to...
Patent
1987-04-13
1989-10-03
Lovering, Richard D.
Plastic and nonmetallic article shaping or treating: processes
Encapsulating normally liquid material
Liquid encapsulation utilizing an emulsion or dispersion to...
264 43, 424 11, 424450, 4284022, 436829, 514 8, 514885, A61K 964, A61K 3722, A61K 4505, B01J 1302
Patent
active
048714887
ABSTRACT:
The present disclosure relates to novel liposome compositions and methods for their preparation. Utilization of the present invention provides an efficient reconstitution of membrane proteins into large (0.1 to 2 micron diameter) phospholipid vesicles with a large, internal aqueous space. The method has been exemplified with the use of glycoproteins of influenza (A/PR8/34) and Sendai (parainfluenza type I) viruses. The method comprises (A) extracting out the desired membrane protein from a source biological material with an extraction buffer comprising a detergent; (B) mixing the extract with a phospholipid solution and deriving a cochleate intermediate; and (C) forming large phospholipid vesicles with integrated membrane protein in a biologically active state.
REFERENCES:
patent: 4148876 (1979-04-01), Almeida et al.
patent: 4196191 (1980-04-01), Almeida et al.
patent: 4201767 (1980-05-01), Fullerton et al.
patent: 4235871 (1980-11-01), Papahadjopoulos et al.
patent: 4261975 (1981-04-01), Fullerton et al.
patent: 4356169 (1982-10-01), Simons et al.
patent: 4416872 (1983-11-01), Alving et al.
patent: 4551228 (1985-11-01), Kelly
patent: 4578269 (1986-03-01), Morein
Scheid et al.: "Identification of Biological, Activities of Paramyxovirus Glycoproteins . . . ", Virology, 57, 475-490(1974), [QR1V5].
Papahadjopoulos et al.: "Cochleath Lipid Cylinders: Formation by Fusion of Unilamellar Lipid Vesicles", Biochimica et Biophysica Acta, 394 (1975), 483-491, [OD1B5].
Fogerite Susan G.
Mannino Raphael J.
Albany Medical College of Union University
Lovering Richard D.
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