Recombination resistant retroviral helper cell and products prod

Details Recombination resistant retroviral helper cell and products prod Recombination resistant retroviral helper cell and products prod

1989-01-12

1992-06-23

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4353201, 4351723, 435236, 435948, 935 32, 935 34, 935 57, 935 70, 935 71, C12N 510

Patent

active

051242630

ABSTRACT:
An improved helper cell for growing up stocks of replication incompetent retrovirus vectors is disclosed. The helper cell resists recombination events due to the fact that natural promoters and poly(A) sequences in the helper sequences have been replaced with foreign promoters and poly(A) sequences bearing little or no homology to the vectors. Plasmids containing these modified sequences can still create a helper cell with resulting expression of the needed helper proteins, yet there is much less risk of recombination events in the helper cell. Also disclosed are vectors produced by such helper cells, target cells infected by such vectors, and helper cells which convert vectors so that they can be used with hosts from different species.

REFERENCES:
patent: 4650764 (1987-03-01), Temin et al.
patent: 4861719 (1989-08-01), Miller
S. K. Chattopadhyay et al. (1981) J. Virology 39:777-791.
Markowitz, D. et al. (1988) Virology 167, 400-406.
J. Embretson et al.; 61 J. Virol. 3454-3462 (1987).
J. Embretson et al.; 61 J. Virol. 2675-2683 (1987).
J. Dougherty et al.; 84 P.N.A.S. USA 1197-1201 (1987).
H. Temin, Retrovirus Vectors For Gene Transfer: Efficient Integration Into And Expression Of Exogenous DNA In Vertebrate Cell Genomes, pp. 149-187, in Gene Transfer (Plenum Press 1986).
M. Emerman et al.; 14 Nuc. Acids Res. 9381-9396 (1986).
J. Dougherty et al.; 168 J. Mol. Cell. Bio. 4387-4395 (1986).
J. Embretson et al.; 60 J. Virol. 662-668 (1986).
K. Wilhelmsen et al.; 52 J. Virol. 172-182 (1984).
M. Emerman et al.; 50 J. Virol 42-49 (1984).
S. Watanabe et al.; 12 J. Mol. Cell. Bio. 2241-2249 (1983).
S. Watanabe et al.; 79 P.N.A.S. USA 5986-5990 (1982).
J. Wilson et al.; 85 P.N.A.S. USA 4421-4425 (1988).
D. Markowitz et al.; 62 J. Virol. 1120-1124 (1988).
B. Davis et al.; 314 Nature 550-553 (1985).
R. Bosselman et al.; 7 J. Mol. Cell. Bio. 1797-1806 (1987).
C. Gorman et al.; 79 P.N.A.S. USA 6777-6781 (1982).
M. Stinski et al.; 55 J. Virol. 431-441 (1985).
J. Sorge et al.; 4 J. Mol. Cell. Bio. 1730-1737 (1984).
A. Miller et al.; 6 J. Mol Cell. Bio. 2895-2902 (1986).
M. Eglitis et al.; 6 BioTechniques 608-614 (1988).
J. Knutson et al.; 164 Anal. Biochem. 44-52 (1987).
S. Kawai et al.; 4 J. Mol. Cell. Bio. 1172-1174 (1984).
P. Southern et al.; 1 J. Mol. App. Gen. 327-341 (1982).
R. Roberts; 15 Nuc. Acids Res. 189-217 (1987).
M. Wickens et al.; 226 Science 1045-1051 (1984).
C. Yanisch-Perron et al.; 33 Gene 103-119 (1985).

Affiliated with

Also associated with

Rating

Recombination resistant retroviral helper cell and products prod does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombination resistant retroviral helper cell and products prod, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombination resistant retroviral helper cell and products prod will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-932548