Chemistry: molecular biology and microbiology – Vector – per se
Patent
1999-01-20
2000-11-07
Schwartzman, Robert A.
Chemistry: molecular biology and microbiology
Vector, per se
435325, 536 23, 536 241, C12N 1563, C12N 1568, C07H 2104
Patent
active
061435570
ABSTRACT:
Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
REFERENCES:
patent: 4673640 (1987-06-01), Backman
patent: 4743546 (1988-05-01), Backman et al.
patent: 4959317 (1990-09-01), Sauer
patent: 5227288 (1993-07-01), Blattner
patent: 5354668 (1994-10-01), Auerbach
patent: 5434066 (1995-07-01), Bebee et al.
patent: 5470727 (1995-11-01), Mascarenhas et al.
patent: 5591609 (1997-01-01), Auerbach
patent: 5614389 (1997-03-01), Auerbach
patent: 5658772 (1997-08-01), Odell et al.
patent: 5677170 (1997-10-01), Devine et al.
patent: 5677177 (1997-10-01), Wahl et al.
patent: 5710248 (1998-01-01), Grose
patent: 5723765 (1998-03-01), Oliver et al.
patent: 5733733 (1998-03-01), Auerbach
patent: 5733743 (1998-03-01), Johnson et al.
patent: 5744336 (1998-04-01), Hodges et al.
patent: 5776449 (1998-07-01), Baum
patent: 5830707 (1998-11-01), Bushman
patent: 5837242 (1998-11-01), Holliger et al.
patent: 5843772 (1998-12-01), Devine et al.
patent: 5851808 (1998-12-01), Elledge et al.
patent: 5858657 (1999-01-01), Winter et al.
patent: 5871907 (1999-02-01), Winter et al.
patent: 5874259 (1999-02-01), Szybalski
patent: 5888732 (1999-03-01), Hartley et al.
patent: 5916804 (1999-06-01), Bushman
patent: 5919676 (1999-07-01), Graham et al.
patent: 5928914 (1999-07-01), Leboulch et al.
patent: 5989872 (1999-11-01), Luo et al.
patent: 6010884 (2000-01-01), Griffiths et al.
Bernard, P., "Positive Selection of Recombinant DNA by CcdB," BioTechniques 21:320-323 (Aug. 1996).
Jaffe, A., et al., "Effects of the ccd Function of the F Plasmid on Bacterial Growth," J. Bacteriol. 163:841-849 (1985).
Nash, H.A., "Integrative Recombination of Bacteriophage Lambda DNA In Vitro," Proc. Natl. Acad. Sci. USA 72:1072-1076 (1975).
Andrews, B.J., et al., "The FLP Recombinase of the 2.mu. Circle DNA of Yeast: Interaction with Its Target Sequences," Cell 40:795-803 (1985).
Andrews, B.J., et al., "Interaction of the FLP Recombinase of the Saccharomyces cerevisiae 2 .mu.m Plasmid with Mutated Target Sequences," Mol. Cell. Biol. 6:2482-2489 (1986).
Reed, R.R., and Grindley, N.D.F., "Transposon-Mediated Site-Specific Recombination in Vitro: DNA Cleavage and Protein-DNA Linkage at the Recombination Site," Cell 25:721-728 (1981).
Reed, R.R., "Transposon-Mediated Site-Specific Recombination: A Defined in Vitro System," Cell 25:713-719 (1981).
Anton, M. and Graham, F. L., "Site-Specific Recombination Mediated by an Adenovirus Vector Expressing the Cre Recombinase Protein: a Molecular Switch for Control of Gene Expression," J. Virol. 69:4600-4606 (Aug. 1995).
Ausubel, F. M., et al., "Mutagenesis by the Polymerase Chain Reaction," in Current Protocols in Molecular Biology, John Wiley & Sons, Inc., Boston, MA, (1995).
Bayley, C. C., et al., "Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system," Plant Mol. Biol. 18:353-361 (1992).
Bernard, P. and Couturier, M., "Cell Killing by the F Plasmid Ccdb Protein Involves Poisoning of Dna-topoisomerase II Complexes," J. Mol. Biol. 226:735-745 (1992).
Betz, U. A. K., et al., "Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination," Curr. Biol. 6:1307-1316 (Oct. 1996).
Bhandari, P and Gowrishankar, J., "An Escherichia coli host Strain Useful for Efficient Overproduction of Cloned Gene Products with Nacl as the Inducer," J. Bacteriol. 179:4403-4406 (Jul. 1997).
Chapin, S.J., et al., "Differential Expression of Alternatively Spliced Forms of MAP4: a Repertoire of Structurally Different Microtubule-binding Domains," Biochem. 34:2289-2301 (Feb. 1995).
Craig, N.L. and Nash, H.A., "The Mechanism of Phage Lambda Site-specific Recombination: Site-specific Breakage of Dna by Int Topoisomerase," Cell 35:795-803 (1983).
Dale, E. C. and Ow, D. W., "Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase," Gene 91:79-85 (1990).
Dale, E. C. and Ow, D. W., "Gene transfer with subsequent removal of the selection gene from the host genome," Proc. Natl. Acad. Sci. USA 88:10558-10562 (1991).
Dang, D. T. and Perrimon, N., "Use of a Yeast Site-Specific Recombinase to Generate Embryonic Mosaics in Drosophila," Develop. Genetics 13:367-375 (1992).
Dymecki, S. M., "A modular set of Flp, FRT and lacZ fusion vectors for manipulating genes by site-specific recombination," Gene 171:197-201 (Jun. 1996).
Fiering, S., et al., "An `in-out` strategy using gene targeting and FLP recombinase for the functional dissection of complex DNA regulatory elements: Analysis of the .beta.-globin locus control region," Proc. Natl. Acad. Sci. USA 90:8469-8473 (1993).
Golic, K. G. and Lindquist, S., "The FLP Recombinase of Yeast Catalyzes Site-Specific Recombination in the Drosophila Genome," Cell 59:499-509 (1989).
Kozak, M., "Comparison of Initiation of Protein Synthesis in Procaryotes, Eucaryotes, and Organelles," Microbiol. Rev. 47:1-45 (1983).
Kozak, M., "An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs," Nucl. Acids Res. 15:8125-8132 (1987).
Kozak, M., "Structural features in eukaryotic mRNAs that modulate the initiation of translation," J. Biol. Chem. 266:19867-19870 (1991).
Kuhn, R., et al., "Inducible Gene Targeting in Mice," Science 269:1427-1429 (Sep. 1995).
Lander, E.S., "The New Genomics: Global Views of Biology," Science 274:536-539 (Oct. 1996).
Matsuzaki, H., et al., "Chromosome Engineering in Saccharomyces cerevisiae by Using a Site-Specific Recombination System of a Yeast Plasmid," J. Bacteriol. 172:610-618 (1990).
McCarthy, JE and Brimacombe, R., "Prokaryotic translation: the interactive pathway leading to initiation," Trends Genet. 10:402-407 (Nov. 1994).
Miki, T. et al., "Control of Segregation of Chromosomal DNA by Sex Factor F in Escherichia coli. Mutants of DNA Gyrase Subunit a Suppress letd (ccdb) Product Growth Inhibition," J. Mol. Biol. 225:39-52 (1992).
Mizuuchi, M. and Mizuuchi, K., "The extent of DNA sequence required for a functional bacterial attachment site of phage lambda," Nucl. Acids Res. 13:1193-1208 (1985).
Nash, H.A. and Robertson, CA, "Purification and Properties of the Escherichia coli Protein Factor Required for .LAMBDA. Integrative Recombination," J. Biol. Chem. 256:9246-9253 (1981).
Nash, H.A., "Bending and supercoiling of DNA at the subsequent site of bacteriophage .LAMBDA.," Trends Biochem. Sci. 15:222-227 (1990).
Nunes-Duby, S.E. et al., "Similarities and differences among 105 members of the Int family of site-specific recombinases," Nucl. Acids Res. 26:391-406 (Jan. 1998).
Orban, P. C., et al., "Tissue- and site-specific DNA recombination in transgenic mice," Proc. Natl. Acad. Sci. USA 89:6861-6865 (1992).
Pichel, J. G., et al., "Timing of SV40 oncogene activation by site-specific recombination determines subsequent tumor progression during murine lens development," Gene 8:3333-3342 (1993).
Sadowski, P.D., "The Flp Recombinase of the 2-microns Plasmid of Saccharomyces cerevisiae," Prog. Nucl. Acid Res. Mol. Biol. 51:53-91 (1995).
Sauer, B., "Functional Expression of the cre-lox Site-Specific Recombination System in the Yeast Saccharomyces cerevisiae," Mol. Cell Biol. 7:2087-2096 (1987).
Sauer, B., "Manipulation of Transgenes by Site-Specific Recombination: Use of Cre Recombinase," Meth. Enzymol. 225:890-900 (1993).
Sauer, B., "Inducible Gene Targeting in Mice Using the Cre/lox system," Methods 14:381-392 (Apr. 1998).
Segall, A. M. and Nash, H. A., "Synaptic intermediates in bacteriophage lambda site-specific recombination: integrase can align pairs of attachment sites," EMBO J. 12:4567-4576 (1993).
Sheffield, P. et al., "Overcoming Expression and Purification Problems of RhoGDI Using a Family of "Parallel" Expression Vectors," Prot. Expr. Purif. 15:34-39 (Feb. 1999).
Smith, A. J. H., et al., "A site-directed chromosomal translocation induced in embryonic stem cells by Cre-loxP rec
Brasch Michael A.
Hartley James L.
Life Technologies Inc.
Sandals William
Schwartzman Robert A.
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