Recombinase mediated DNA therapies

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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435325, 536 231, C12N 1566

Patent

active

059290439

ABSTRACT:
Nucleoproteins comprising overlapping homologous DNA sequences coated with a recombinase are employed for introduction into cells to produce double D-loop structures. The sequences may be modified with various moieties which result in modification of DNA. Formation of the D-loops results in inhibition of replication and transcription, where the moieties can be employed for permanent scission or modification of the DNA. The methods and compositions can be used for investigating physiological processes, for producing animal models, and for inhibition of an undesirable phenotype in vivo.

REFERENCES:
patent: 5264618 (1993-11-01), Felgner et al.
patent: 5273881 (1993-12-01), Sena et al.
Revet, et al., Homologous DNA Targeting with RecA Protein-Coated Short DNA Probes and Electron Microscope Mappingon Linear Duplex Molecules (1993) Reprinted from J. Mol. Biol. 232:779-791.
Sena, et al., Targeting in Linear DNA Duplexes with Two Complementary Probe Strands for Hybrid Stability (Apr. 1993) Nature Genetics, pp. 365-372.

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