Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2006-11-07
2006-11-07
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S320100
Reexamination Certificate
active
07132290
ABSTRACT:
In the present invention, viruses, plasmids or both are constructed which contain viral DNA and lox sites positioned such that site-specific recombination between lox sites in separate plasmids results in generation of infectious viral DNA at high-efficiency in cotransfected host cells that have been engineered to express the Cre recombinase. Because of the high-efficiency and specificity of the Cre enzyme, suitably engineered plasmids can be readily recombined to produce infectious virus at high-efficiency in cotransfected 293 cells, without, at the same time, producing wild-type adenovirus, with the attendant problems for removal thereof. Use of recombinases besides Cre and recombinase recognition sites besides lox sites, and use of cells other than 293 cells are also disclosed and enabled, as are kits incorporating the site-specific vector system.
REFERENCES:
patent: WO 97/25446 (1997-07-01), None
Bett et al., An efficient and flexible system for construction of adenovius vectors with insertions or deletions in early regions 1 and 3, Sep. 1994, Proc. Natl. Acad. Sci. USA, vol. 91, pp. 8802-8806.
Graham Frank L.
Ng Philip
Parks Robin J.
AdVec, Inc.
Beusse Wolter Sanks Mora & Maire PA
Fischer Joseph
Vogel Nancy
LandOfFree
Recombinase-based system for combustion of adenovirus vectors does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinase-based system for combustion of adenovirus vectors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinase-based system for combustion of adenovirus vectors will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3638853