Recombinant viral vector system

Chemistry: molecular biology and microbiology – Vector – per se

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Details

435 691, 4351723, 536 231, 536 241, C12N 1586, C12N 1511

Patent

active

054787459

ABSTRACT:
The present invention relates to a system for replication and encapsidation of recombinant DNA fragments into virus particles comprised of adenovirus associated viral (AAV) capsid proteins. The invention provides a means of obtaining recombinant viral stocks that may be used to treat patients suffering from genetic diseases.

REFERENCES:
patent: 4797368 (1989-01-01), Carter et al.
patent: 5139941 (1992-08-01), Muzyczka et al.
Cheung, A. K. et al., 1980, "Integration of the Adeno-Associated Virus Genome into Cellular DNA in Latently Infected Human Detroit 6 Cells" J. Virology 33:739-748.
Berns, K. I. et al., 1982, "Adeno-Associated Virus Latent Infection" in Virus Persistence, eds. Mahey et al. (Cambridge University Press) pp. 249-265.
Samulski, R. J. et al., 1982, "Cloning of Adeno-Associated Virus Into pBR322: Rescue of Intact Virus From the Recombinant Plasmid in Human Cells", Proc. Natl. Acad. Sci. U.S.A. 79:2077-2081.
Miller A. D., 1990, "Retrovirus Packaging Cells" Human Gene Therapy 1:5-14.
Kotin, R. M. et al., 1990, "Targeted Integration by Adeno-Associated Virus", Proc. Natl. Acad. Sci. U.S.A. 87:2211-2215.
Samulski, R. J. et al., 1991, "Targeted Integration of Adeno-Associated Virus (AAV) Into Human Chromosome 19" The EMBO J. 10:3941-3950.
Muzyczka, N., 1992, "Use of Adeno-Associated Virus as a General Transduction Vector for Mammalian Cells" Current Topics in Microbiology & Immunology 158:97-129.

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