Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
1995-11-13
2001-05-08
Caputa, Anthony C. (Department: 1642)
Chemistry: molecular biology and microbiology
Vector, per se
Reexamination Certificate
active
06228637
ABSTRACT:
BACKGROUND OF THE INVENTION
I. Field of the Invention
This invention relates to a recombinant vector which can confer upon potato plants immunity against potato virus Y necrosis line (hereinafter also referred to as “PVY-T”). This invention also relates to a method for conferring immunity against PVY-T upon potato plants and to a potato plant having immunity against PVY-T.
II. Description of Related Art
PVY-T causes tobacco yellow spot necrosis which causes severe necrosis in tobacco. PVY-T is transferred by aphids. If potato plants infected with PVY-T are cultivated, PVY-T may be transferred by aphids, thereby causing severe damage to tobacco. Although PVY-T may cause mosaic disease in potato plants, the symptoms are slight and unclear in a number of varieties, so that it is difficult to remove the diseased plants during cultivation of seed potatoes. Therefore, the diseased potato plants may serve as a source of PVY-T.
With some potato varieties, inoculation of PVY-T causes necrosis spots in the inoculated leaf in the present generation, while in the next generation, symptoms are not observed and infection by PVY-T can not be detected. If these potato varieties are bred by cross breeding, PVY-T-resistant potato plants may be obtained. However, it is expected that it takes as long as 10 years or more to obtain a variety resistant to PVY-T by this method. If resistance to PVY-T can be conferred upon potato plants by genetic engineering techniques, PVY-T-resistant potato plants can be obtained in a short period of time, which is very advantageous.
A method for conferring upon a plant resistance against a virus is known, in which the gene encoding the coat protein (hereinafter also referred to as “CP”) of the virus is introduced to the plant (Japanese Laid-open Patent Application (Kokai) Nos. 62-201527 and 62-285791; Plant Cell Technology Vol. 4, No. 2 (1992) pp.101-109, Bio/Technology 8, 127-134 (1990)). According to this method, although resistance against the virus is conferred upon the plant, the plant produces the CP of the virus. If a plant produces a virus CP, amino acids and energy are consumed for the production of virus CP, so that the growth of the plant is not as good as other plants which do not produce the virus CP. Further, in a plant such as potato which is used as a food, the safety of the CP with respect to health must be investigated. Thus, if a plant which is resistant to a virus does not produce the virus CP is obtained, it is very advantageous.
SUMMARY OF THE INVENTION
Accordingly, an object of the present invention is to provide a recombinant vector by which immunity against PVY-T may be conferred upon potato plants without making the plants produce the CP of PVY-T. Another object of the present invention is to provide a method for conferring upon potato plants immunity against PVY-T. Still another object of the present invention is to provide a potato plant having immunity against PVY-T.
The present inventors intensively studied to find that immunity against PVY-T may be conferring upon potato plants without making the potato plants produce the CP of PVY-T by transforming the potato plants with a recombinant vector containing a gene encoding the CP of PVY-T (SEQ ID NO:1) at a downstream region of the leader sequence of RNA4 (SEQ ID NO:4) of cucumber mosaic virus (hereinafter also referred to as “CMV”), thereby completing the present invention.
That is, the present invention provides a recombinant vector comprising a promoter which functions in a potato plant cell; an operably linked leader sequence of RNA4 of CMV (SEQ ID NO:4), which is located downstream of said promoter; and an operably linked sequence which encodes a CP of PVY-T, (SEQ ID NO:1) which is located downstream of said leader sequence; said recombinant vector being capable of transforming potato plants. The present invention also provides a method for conferring immunity against PVY-T upon a potato plant, comprising transforming said potato plant with said recombinant vector according to the present invention. The present invention still further provides a potato plant having immunity against PVY-T, which was prepared by the method according to the present invention.
By the present invention, a potato plant having immunity against PVY-T, which does not produce CP of PVY-T, was first provided. Therefore, the present invention is effective for the prevention of tobacco yellow spot necrosis and other diseases of potato plants in which PVY-T participates.
REFERENCES:
patent: 4970168 (1990-11-01), Tumer
patent: 806481 (1997-11-01), None
patent: WO8905858 (1989-06-01), None
patent: WO8912100 (1989-12-01), None
patent: WO9002184 (1990-03-01), None
Barker et al (1984) Ann Appl. Biol 105 (3):539-546.*
Hidaka et al (1985) J. Biochem 97:161-171.*
Hay et al (1989) Arch virol 107:111-122.*
Nejidat et al (1990) Physiol. Plant. 80:662-668.*
Farinelli et al Bio/Technology vol. 10 1020-1025, Sep. 1992.*
Clark et al. “engineering virus resistance in transgenic plants”. Plant Biol. vol. 11, pp 273-283, 1990.*
Cuozzo et al. “Virual protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA”. Bio/Technology. vol. 5, No. 6, pp 549-554, 557, 1988.*
Kawchuk et al. “Sense and antisense RNA-mediated resistance to potato leafroll virus in Russet Burbank potato plants”. Mol. Plant-Microbe Interact. vol. 4, No. 3, pp 247-253, 1991.*
Hull et al. “Approaches to nonconventional control of plant virus diseases”. Crit. Rev. Plant. Sci 11. vol. 1, pp 17-33, 1992.*
N.Shigetou (1992)Plant Cell Technology4 (2):101-109.
A. Inoue (1991)Ann. Phytopath. Soc. Japan57:615-622.
N. Nitta et al. (1988)Ann. Phytopath. Soc. Japan54:516-522.
G. An et al. (1988)Plant Molecular Biology ManualA3:1-19.
Udagawa et al. (1972)Japan Plant Pathology Assoc. Report38 (3):210, Ab. 150.
P. S. Thomas (1980)Proc. Natl. Acad. Sci. USA77 (9):5201-5205.
R. Van der Vlugt et al. (1989)J. Gen. Virol.70:229-233.
C. Lawson et al. (1990)Bio/Technology8:127-134.
L. Farinelli et al. (1992)Bio/Technology10:1020-1025.
S. Hidaka et al. (1985)J. Biochem.97:161-171.
Hayashi Yumiko
Kadotani Naoto
Kasaoka Keisuke
Kuwata Shigeru
Birch & Stewart Kolasch & Birch, LLP
Brumback Brenda G.
Caputa Anthony C.
Japan Tobacco Inc.
LandOfFree
Recombinant vector, method for giving immunity against PVY-T... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant vector, method for giving immunity against PVY-T..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant vector, method for giving immunity against PVY-T... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2486816