Recombinant vector having a Streptomyces achromogenes DNA sequen

Details Recombinant vector having a Streptomyces achromogenes DNA sequen Recombinant vector having a Streptomyces achromogenes DNA sequen

1991-01-08

1993-08-31

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 712, 4351723, 43525235, C12N 1563, C12N 1569, C12N 121

Patent

active

052408582

ABSTRACT:
An improved means for amplifying DNA sequences is disclosed. The invention provides a means for creating tandemly repeated gene sequences. One attaches a selected foreign gene sequence in a S. achromogenes fragment and then exposes the recombinant segment to antibiotic challenge. Temperature sensitive plasmids or other curable vectors can be used to assist in the amplification and the positioning of the resulting gene directly in chromosomal host DNA.

REFERENCES:
J. Schottel et al., 146 J. Bacteriol. 360-368 (1981) (M252 variant of S. lividans 1326).
R. Freeman et al., 98 J. Gen. Microl. 453-465 (1977) (chloramphenicol-sensitive strains).
J. Altenbuchner et al., 195 Mol. Gen. Genet. 134-138 (1984) (chloramphenicol-sensitive strains).
U. Hornemann et al., J. Cell Biol. 15th Annual Meeting, Abstract 1982 (1986).
U. Hornemann et al., 5th International Symposium On The Genetics Of Industrial Organisms, Abstract S4-P2 p. 29 (1986).
A. Bertinuson et al., 1984 Meeting Of The American Society For Microbiiology, Abstract Submission Form (abstract published at meeting) (admitted prior art).
C. Otto et al., Wind River Conference On Genetic Exchange, p. 36 (1985).
J. Altenbuchner et al., 5 Bio/Technology 1328-1329 (1987).
J. Altenbuchner et al., 201 Mol. Gen. Genet. 192-197 (1985).
A. Birch et al., 131 J. Gen. Microb. 1299-1303 (1985).
U. Hornemann et al., 169 J. Bacteriol. 2360-2366 (1987) (not prior art).
E. Katz et al., 129 J. Gen. Microb. 2703-2714 (1983).
D. Hopwood et al., 129 J. Gen. Microb. 2257-2269 (1983).
M. Bibb et al., 41 Gene E357-E368 (1986).
C. Thompson et al., 20 Gene 51-62 (1982).
Y. Potekhin et al., 19 Molecular Biology (Russian) 805-817 (1985).
K. Koller et al., 6th International Sym. Biol. Actin, Abstract L71 (1985).
B. Peterson et al., 161 J. Bacteriol. 1042-1048 (1985).
B. Bhuyan et al., Rubradirin, A New Antibiotic, Antimicrob. Agents Chemother. 91-96 (1964).
H. Motamedi et al., 84 P.N.A.S. U.S.A. 4445-4449 (1987).
M. Mori et al., 134 J. Gen. Microb. 85-95 (1988) (not prior art).

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