Recombinant vector and use thereof for exocellular preparation o

Details Recombinant vector and use thereof for exocellular preparation o Recombinant vector and use thereof for exocellular preparation o

1994-03-08

1997-10-07

Schwadron, Ronald B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 712, 4351723, 43525231, 4352525, 4353201, 435839, 536 2353, 536 231, 536 237, 536 241, 5303873, 530867, 53038824, 530808, 935 22, 935 29, 935 48, 935 74, C12P 2108, C12N 1563, C12N 1513, C12N 1575

Patent

active

056747128

ABSTRACT:
A recombinant vector for the expression and secretion of antibodies in single molecule form (scFv) from B. subtilis, where said vector comprises the promoter of the gene for neutral protease, a new secretion sequence (I) and a DNA sequence coding a scFv antibody of interest, a strain of B. subtilis transformed with said recombinant vector, and a process for the exocellular production of scFv antibodies by culturing said strain of B. subtilis are described. The recombinant vector allows the expression of scFv in a completely soluble form and its secretion in high yields.

REFERENCES:
Critical Reviews in Biotechnology, vol. 12, Issues 5,6, 1992, pp. 437-462, J.S. Sandhu, "Protein Engineering of Antibodies".

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