Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1996-07-16
1999-12-28
Guzo, David
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
43525233, 4353201, 536 231, 536 237, C12N 121, C12N 1570, C12N 1511
Patent
active
060080515
ABSTRACT:
The present invention is directed to a recombinant vector capable of directing the synthesis of gas vesicles in a non-floating cells. Cells transformed by the vector of the invention are also provided. The present invention is further directed to a method for conferring buoyancy to non-floating cells. The method of the instant invention is useful in harvesting cells, for example in fermentation processes, and in bioremediation, for example in conferring buoyancy to hydrocarbon-degrading bacteria.
REFERENCES:
Blaseio et al. "Transformation of Halobacterium halobium: Development of Vectors and Investigation of Gas Vesicle Synthesis" PNAS, vol. 87, pp. 6772, 6776, Sep. 1990.
Jones et al. "Structure and Organization of the Gas Vesicle Gene Cluster on the Halobacterium halobium plasmid PNRC 100." Gene, vol. 102 (1991) pp. 117-122.
DasSarma et al. (1987) "A Plasmid-Encoded Gas Vesicle Protein Gene in a Halophilic Archaebacterium" Molecular Microbiology 1, 3650370.
DasSarma et al. (1988) "High-Frequency Mutations in a Plasmid-Encoded Gas Vesicle Gene in Halobacterium Halobium", Proc. Natl. Acad. Sci. USA 85, 6861-6865.
Horne et al. (1988) "Two Genes Encoding Gas Vacuole Proteins in Halobacterium Halobium", Mol. Gen. Genet. 213, 459-464.
Horne et al. (1991) "A DNA Region of 9kbp Contains all Genes Necessary for Gas Synthesis in Halophilic Archaebacteria", Molecular Microbiology 5, 1159-1174.
Jones et al. (1989) "Analysis of Insertion Mutants Reveals Two New Genes in the pNRC100 Gas Vesicle Gene Cluster of Halobacterium Halobium", Nucleic Acids Research 17, 7785-7793.
DasSarma Shiladitya
Halladay John
Ng Wai-lap
Guzo David
University of Massachusetts
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