Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-05-16
2006-05-16
Mosher, Mary E. (Department: 1648)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S235100, C435S091400, C435S091410, C435S069300
Reexamination Certificate
active
07045313
ABSTRACT:
Methods and compositions are provided for the use of vaccinia virus or other poxviruses as vectors for expression of foreign genes. Expression of foreign genes is obtained by combining vaccinia virus transcriptional regulatory sequence with uninterrupted foreign protein coding sequences in vitro to form a chimeric gene. The chimeric gene is flanked by DNA from a non-essential region of the vaccinia virus genome to provide sites for in vivo homologous recombination. These steps are facilitated by the construction of plasmids that contain multiple restriction endonuclease sites, next to the vaccinia transcriptional regulatory sequences, for insertion of any foreign protein coding sequence. Transfection procedures are used to introduce the DNA into cells where homologous recombination results in the insertion of the chimeric gene into a non-essential region of the vaccinia virus genome. Infectious vaccinia virus recombinants are distinguished or selected by expression of the foreign gene, loss of activity of a vaccinia virus gene, or by DNA—DNA hybridization. Expression of the foreign gene is obtained by infecting cells or animals with the recombinant vaccinia virus. Examples are provided to show expression of prokaryotic, RNA virus and other DNA virus genes in vaccinia recombinants.
REFERENCES:
patent: 4603112 (1986-07-01), Paoletti et al.
patent: 4722848 (1988-02-01), Paoletti et al.
patent: 4769330 (1988-09-01), Paoletti et al.
patent: 5110587 (1992-05-01), Paoletti et al.
patent: 5155020 (1992-10-01), Paoletti
patent: 5174993 (1992-12-01), Paoletti
patent: 5204243 (1993-04-01), Paoletti
patent: 5225336 (1993-07-01), Paoletti
patent: 5266313 (1993-11-01), Esposito et al.
patent: 5338683 (1994-08-01), Paoletti
patent: 5364773 (1994-11-01), Paoletti et al.
patent: 5494807 (1996-02-01), Paoletti et al.
patent: 5505941 (1996-04-01), Paoletti
patent: 5583028 (1996-12-01), Paoletti et al.
patent: A-0 083 286 (1983-07-01), None
patent: A-0 284 416 (1988-09-01), None
patent: A-0 338 807 (1989-10-01), None
patent: 0397580 (1990-11-01), None
patent: WO 89/03429 (1989-04-01), None
patent: WO 89/12684 (1989-12-01), None
patent: WO 93/25666 (1993-12-01), None
Cheng et al J.of Virology 1986 vol. 60 p 337-344.
Solnick, D. 1981.Cellvol. 24 p. 135-143.
Subramani, S et al. 1981.Molec. Cell. Biolvol. 1 p. 845-864.
Homer, D. H et al., 1979.Cellvol. 17 p. 725-735.
Venkatesan, S. et al. 1981.Cellvol. 125 p. 805-813.
Moriarty, A.M. et al. 1981.Proc. Nat. Acad. Sci. USAvol. 78 p. 2606-2610.
Paoletti, E. et al, 1980,J. Virolvol 33 p. 208-219.
Witlek, R. et al. 1980.Cellvol 21 p. 487-493.
Webster's New World Dictionary of the American Language, College Edition. 1968. The World Publishing Company, Cleveland and New York p.18.
Weir et al.,Proc.Nat'l Acad. Sci. USA79: 1210-14 (1982).
Paicali & Paoletti,Proc. Nat'l Acad. Sci. USA79: 4927-31 (1982).
Panicali et al.,Proc. Nat'l Acad. Sci. USA80: 5364-68 (1983).
Panoletti et al.,Proc. Nat'l Acad. Sci. USA81: 193-97 (1984.
Moss, “Poxyiridae and Their Replication” in Virology 2079-2111 (Raven Press 1990).
Hanafusa et al.,Biken's J.2: 77-82 (1959.
Fenner et al.,Virology11: 185-201 (1960).
Boyle and Coupar,J. gen Virol.67: 1591-1600 (1986).
Boyle and Coupar,Virus Res.10: 343-56 (1988).
Taylor et al.,Vaccine6: 497-503 (1988).
Spehner et al.,J. Virol.64: 527-33 (1990).
Post & Roizman, “A generalized technique for deletion of specific genes in large genomes: gene 22 of herpes simplex virus is not essential for growth,”Cell25: 227-32 (1981).
Peabody et al., “Effect of upstream reading frames on translation efficiency in similan virus 40 recombinants,” translation efficiency in simian virus 40 recombinants,Molec.&Cell, Biol.6: 2704-11 (1986).
Perez et al., “Expression of the Rous sarcoma virusenvgene from a simian virus 40 late-region replacement vector: Effects of upstream initiation codons,”J. Virol.61: 1276-81 (1987).
Geballe & Mocarski, “Translational control of cytomegalovirus gene expression is mediated by upstream AUG condons,”J. Virol.62: 3334-40 (1988).
Hensel et al., “Effects of alterations in the leader sequence of Rous Sarcoma virus RNA on initiation of translatioin,”J. Virol.63: 4986-90 (1989).
Davison & Moss, “The structure of vaccinia virus early promoters,”J. Mol. Biol.210: 749-69 (1989).
McCarthy et al. “Isolation and Characterization of Entomopox Virions from Virus-Containing Inclusions ofAmsacia moorei”, Virology 59: 59-69 (1974).
Munyon et al. “Transfer of Thymidine Kinase to Thymidine Kinaseless L Cells by Infection with Ultraviolet-Irradiated Herpes Simplex Virus”,J. Virology,813-820 (Jun. 1971).
Munyon et al. “Biochemical Transformation of L-Cells with Ultraviolet-Irradiated Herpes Simplex Virus”,Federation Proceedings,31(6) (Nov.-Dec. 1972).
Sam & Dumbell “Expression of Poxvirus DNA in Coinfected cells and Marker Rescue of Thermosensitive Mutants by Subgenomic Fragments of DNA,”Ann. Virol.(Inst. Pasteur), 132 E. 135-150 (1981).
Rosel, J., et al., “Transcriptional and Translational Mapping and Nucleotide Sequence Analysis of a Vaccinia Virus Gene Encoding the Precursor of the Major Core Polypeptide 4b”,J. Virol.56, 830-838 (1985).
Rosel, J., et al., “Conserved TAAATG Sequence at the Transcriptional and Translational Initiation Sites of Vaccinia Virus Late Genes Deduced by Structural and Functional Analysis of the HindIII H Genome Fragment”,J.Virol.60, 436-449 (1986).
Venkatesan, S. et al., “Distinctive Nucleotide Sequence Adjacent to Multiple Initiation and Termination Sites of an Early Vaccinia Virus Gene”,Cell125, 805-813 (1981).
Venkatesan, S., et al., “Complete Nucleotide Sequence of Two Adjacent Early Vaccinia Virus Genes Located within the Inverted Terminal Repetition”,J. Virol.44, 637-646 (1982).
Weaver, R.F., et al., “Mapping of RNA by a Modification of the Berk-Sharp Procedure: The 5′ Terminal of 15S b-globin mRNA Precustor and Mature 10S b-glovinin mRNA Have Identical Map Coordinates”,Nucl. Acids Res.7, 1175-1193 (1979).
Weir, J.P. and Moss B., “Regulation of Expression and Nucleotide Sequence of a Late Vaccinia Virus Gene”,J.Virol.51, 662-669 (1984).
Jonathan A. Cooper, et al., “Hybridization Selection and Cell-Free Translation of mRNA's Encoded Within the Inverted Terminal . . . ”,J. Virol37, 284-294 (1981).
Jonathan A. Cooper, et al., “Extension of the Transcriptional and Translational Map of the Left End of the Vaccinia Virus . . . ”,J. Virol.39, 733-745 (1981).
Sundararajan Venkatesan, “In Vitro Transcription of the Inverted Terminal Repetition of the Vaccinia Virus Genome: Correspondence of Initiation . . . ”,J. Virol37, 738-747 (1981).
R.K. Kent, “Isolation and Analysis of the Vaccinia Virus P4B Gene Promoter”,Ph. D. Dissertation, University of Cambridge(1988).
Baldick, C.J., et al., “Mutational Analysis of the Core, Spacer, and Initiator Regions of Vaccine Virus Intermediate-class promoters”,J. Virol.66, 4710-4719 (1992).
Belle, Isle H., et al., “Cell-Free Translation of Early and Late mRNAs Selected by Hybridization . . . ”,Virology112, 306-317 (1981).
Bertholet, C., et al., “One Hundred Base Pairs of 5′ Flanking Sequence of a Vaccinia Virus Late Gene . . . ”,Proc. Nat'l. Acad. Sci. USA,82, 2096-2100 (1985).
Boone, R.F., et al., “Methylated 5′ terminal Sequence of Vaccinia Virus mRNA Spacies Made in Vivo at Early and Late Times After Infection”,Virology79, 67-80 (1977).
Fenner, F. and Woodroffe. G.M., “The Reactivation of Poxviruses. II. The Range of Reactivating Viruses”,Virology11, 185 (1960).
Green, M.R. and Roeder, R.G., Definition of a Novel Promoter for the Adenovirus-Associated Virus mRNA,Cell22, 231-242 (1980).
Hanafusa, H., et al., “Transformation Phenomenon in the Pox Group of Viruses. II. Trans
Mackett Michael
Moss Bernard
Smith Geoffrey
Heller Ehrman LLP
Mosher Mary E.
The United States of America as represented by the Department of
LandOfFree
Recombinant vaccinia virus containing a chimeric gene having... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant vaccinia virus containing a chimeric gene having..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant vaccinia virus containing a chimeric gene having... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3614778