Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1988-11-10
1991-12-31
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
C12N 701
Patent
active
050772139
ABSTRACT:
A recombinant vaccinia virus, effective in combatting hepatitis B infections in humans, is provided. The virus has incorporated therein the entire HBsAg gene, and is capable of expressing in and secreting from animal (including human) cells, in particulate form, all three of the constituent protein epitopes S, preS2 and preS1 constituting the proteinaceous surface antigen of hepatitis B virus. The recombinant vaccinia virus is prepared by recombination of a selected vaccinia virus strain of very low toxicity towards mammals with an expression plasmid containing the HBsAg sequence and the promoter sequence thereof in operative registry therewith, this sequence being provided at a location interposed within a gene sequence common to the expression plasmid and the vaccinia viral genome. This recombinant virus can be used as a novel live vaccine of low toxicity and also as a system for the production of secretable HBsAg particles with all of the three components, which can be applied as a novel sub-unit vaccine.
REFERENCES:
patent: 4442205 (1984-04-01), Hamer et al.
patent: 4769330 (1988-09-01), Paoletti et al.
Cheng et al., J. of Virology, 11/86, vol. 60, pp. 337-344, Hepatitis B Virus Large Surface Protein is not Secreted but is Immunogenic.
Cheng et al., J. of Virology, 4/87, vol. 61, pp. 1286-1290, Selective Synthesis and Secretion of Particles Composed of HBV Middle S Protein.
Paoletti et al., PNAS, 1/84, vol. 81, pp. 193-197, Construction of Live Vaccines Using Genetically Engineered Pox Viruses.
Jawetz et al., Review of Medical Microbiology, 1974, Chpt. 36, p. 419.
Li Zai-Ping
Wang Yuan
Schwartz Richard A.
Shanghai Institute of Biochemistry, Chinese Academy of Sciences
LandOfFree
Recombinant vaccinia virus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant vaccinia virus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant vaccinia virus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1509803