Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2006-10-03
2006-10-03
Patterson, Jr., Charles L. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S320100, C435S252300, C536S023200
Reexamination Certificate
active
07115407
ABSTRACT:
In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme. This one polypeptide possesses two related enzymatic functions; namely an endonuclease activity which recognizes the DNA sequence 5′-TCC(Pu)AC-3′ and cleaves as indicated by the arrows:5′-TCCRAC(N20)↓-3′3′-AGGYTG(N18)↑-5′and a second enzymatic activity that recognizes the same DNA sequence, 5′-TCC(Pu)AC-3′, but modifies this sequence by the addition of a methyl group to prevent cleavage by the MmeI endonuclease activity.
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Bhatia Tanya
Davis Theodore
Lovasco Lindsay
Morgan Richard D.
New England BioLabs, Inc.
Patterson Jr. Charles L.
Strimpel Harriet M.
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