Recombinant thermostable enzyme which releases trehalose from no

Details Recombinant thermostable enzyme which releases trehalose from no Recombinant thermostable enzyme which releases trehalose from no

1995-07-21

1999-01-05

Caputa, Anthony C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435100, 435195, 435200, 435201, 4352533, 435822, 530350, 530825, C12N 924, C12P 1918, C07K 14195

Patent

active

058561463

ABSTRACT:
Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industial scale and in a satisfactorily-high yield.

REFERENCES:
patent: 4521252 (1985-06-01), Miyake et al.
patent: 5472863 (1995-12-01), Maruta et al.
Licia et al, Thermostable Amylolytic Activity fron Sulfolbus solfataricus, vol. No. 4, pp. 201-203, 1991.
Sambrook et al., Molecular Cloning: A Laboratory Manual; Cold Spring Harbour Labratory Press; pp. v-xxxii; 1989.
Laemmli, U.K., "Cleavage of Structural Proteins during the assembly of the Head of Bacteriophage T4."; Nature; vol. 227; pp. 685; 15 Aug. 1970.
The Amylase Research Society of Japan, editors; Handbook of Amylases and Related Enzymes: Their Sources, Isolation Methods, Properties and Applications. Permagon Press; pp. xi-71; 1988.

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