Recombinant thermostable enzyme which releases trehalose from no

Details Recombinant thermostable enzyme which releases trehalose from no Recombinant thermostable enzyme which releases trehalose from no

1997-02-11

2000-02-22

Knode, Marian C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 711, 435 712, 435183, 435200, 43525233, 4353201, 536 232, 536 231, 536 237, C12P 2102, C12N 924, C12N 1531, C12N 1552

Patent

active

060279184

ABSTRACT:
Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.

REFERENCES:
patent: 4521252 (1985-06-01), Miyake et al.
patent: 5591612 (1997-01-01), Meruta et al.
Sambrook et al, Molecular Cloning: A Laboratory Manual; Cold Spring Harbour Laboratory Press; pp. v-xxxii; 1989.
Laemmli, U.K., "Cleavage of Structural Proteins during the Assembly of the Head of Bateriophage T4"; Nature; vol. 227; pp. 680-685; Aug. 15, 1970.
The Amylase Research Society of Japan, editors; Handbook of Amylases and Related Enzymes: Their Sources, Isolation Methods, Properties and Applications; Permagon Press; pp. xi-71; 1988.
Licia et al., Thermostable Amylolytic Activity for Sulfolbus solfataricus; Biotech Forum Europe; vol. 8, No. 4, pp. 201-203; 1991.
Reeck et al 1987 Cell 50 p 667, Aug 28, 1987.

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