Recombinant thermostable enzyme for converting maltose into treh

Details Recombinant thermostable enzyme for converting maltose into treh Recombinant thermostable enzyme for converting maltose into treh

1995-09-29

1998-06-30

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435100, 435200, 43525233, 435325, 435419, 536 232, C12N 924, C12N 1556

Patent

active

057732825

ABSTRACT:
Disclosed are a recombinant thermostable enzyme, which converts maltose into trehalose and is stable up to a temperature of about 80.degree. C. even when incubated at pH 7.0 for 60 min, a preparation of the enzyme, a DNA encoding the enzyme, a recombinant DNA containing the DNA, a transformant, and an enzymatic conversion method of maltose by using the enzyme.

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Sambrook et al. Molecular Cloning: A Laboratory Manual, 2nd Edition, 1989, pp. 11.3-11.19.
Nishimoto et al, "Existence of a Novel Enzyme Converting Maltose into Trehalose", Bioscience Biotechnologh and Biochemistry, vol. 59, No. 11, pp. 2189-2190, 1995.
Nishimoto et al, "Purification and Properties of a Novel Enzyme, Trehalose Synthase, from Pimelobacter sp. R48", Bioscience Biotechnology and Biochemistry, vol. 60, No. 4, pp. 640-644, 1996.
Tsusaki et al, "Cloning and sequencing of trehalose synthase gene from Pimelobacter", Biochimica Et Biophysica Acta, vol. 1290, pp. 1-3, 1996.
Nishimoto et al, "Purificaiton and Characterization of a Thermostable Trehalose Synthase from Thermus aquaticus", Bioscience Biotechnology and Biochemistry, vol. 60, No. 5, pp. 835-839, 1996.
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Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, 2nd Edition, pp. xi-xxxviii (1989).
Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, 2nd Edition, pp. 1.74-1.81 (1989).
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