Recombinant systems for expression of cholera B-subunit with the

Details Recombinant systems for expression of cholera B-subunit with the Recombinant systems for expression of cholera B-subunit with the

1997-01-21

1998-11-10

Ulm, John

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 4353201, 536 234, C12N 121, C12N 1562

Patent

active

058342460

ABSTRACT:
Disclosed herein are procedures whereby with the aid of recombinant DNA methods, the expression of the binding subunit protein of cholera toxin (CTB) or derivatives thereof including hybrid gene fusion proteins to CTB has been brought under the control of a foreign (non-cholera toxin) promoter and/or the CTB protein or its derivatives being synthesized with a foreign rather than the natural leader peptide to facilitate translocation across cell membranes.

REFERENCES:
Hirst et al., P.N.A.S. 81:7752-7756, Dec. 1984.
Dallas et al., Nature 288:499-501, Dec. 4, 1980.

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