Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-07-08
1993-12-07
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 697, 4352523, 4353201, C12N 1531, C12N 1562
Patent
active
052682765
ABSTRACT:
Disclosed herein are procedures whereby with the aid of recombinant DNA methods, the expression of the binding subunit protein of cholera toxin (CTB) or derivatives thereof including hybrid gene fusion proteins to CTB has been brought under the control of a foreign (non-cholera toxin) promoter and/or the CTB protein or its derivatives being synthesized with a foreign rather than the natural leader peptide to facilitate translocation across cell membranes.
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R. W. Old, Principles of Gene Manipulation Studies in Microbiology vol. 2 (Blackwell Scientific Pub; London).
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Nature 306:551-557, 1983, Mekalanos et al. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development.
J. Biol. Chem. 258:13722-13726, Nov. 25, 1983, Lockman et al. Nucleotide Sequence Analysis of the A2 and B Subunits of Vibrio cholerae Enterotoxin.
J. Immun. 133:1818-1824, Oct. 4, 1984, McKenzie et al. Cholera Toxin B Subunit as a Carrier Protein to Stimulate a Mucosal Immune Response.
Infect. & Immun. 56:2144-2148, Aug. 1988, Okamoto et al. Reduction of Enterotoxic Activity of Escherichia coli Heat Stable Enterotoxin by Substitution by Substitution for an Asp. Residue.
P.N.A.S. 80:21-25, Jan. 1983, DeBoer et al. The tac promoter: A functional hybrid derived from the trp and lac promoters.
J. Mol. Biol. 189:113-130, 1986, Studier et al. Use of Bacteriophage T7 RNA Polymerase to Direct Selective High-level Expression of Cloned Genes.
Holmgren Jan
Sanches Castillo Joaquin
Hill Jr. Robert J.
Ulm John D.
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