Recombinant streptavidin-protein chimeras useful for conjugation

Details Recombinant streptavidin-protein chimeras useful for conjugation Recombinant streptavidin-protein chimeras useful for conjugation

1991-07-12

1994-07-12

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

5303911, 5303915, 536 221, 536 231, 536 232, 536 234, 536 237, 435 71, 4352523, 435 691, 4353201, C07K 300, C07H 2104, A61K 3514, C12Q 100

Patent

active

053289854

ABSTRACT:
A novel recombinant streptavidin-protein A chimeric protein which allows conjugation of antibody molecules with biological materials. The chimeric protein is efficiently expressed in Escherichia coli and is purified by simple procedures. The purified chimetic protein can bind one biotin molecule and one to two immunoglobulin molecules per subunit.

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patent: 5100788 (1992-03-01), Lofdahl
patent: 5109124 (1992-04-01), Ramachandran
patent: 5168049 (1992-12-01), Meade
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Sano, Takeshi and Cantor, Charles, A Streptavidin-Protein A Chimera that Allows One-Step Production of a Variety of Specific Antibody Conjugates, Biotechnology, vol. 9, pp. 1378-1391, (Dec. 1991).

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