Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2005-06-28
2005-06-28
Achutamurthy, Ponnathapu (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S006120, C435S440000, C435S252300, C435S320100, C536S063000
Reexamination Certificate
active
06911330
ABSTRACT:
A secoisolanciresinol dehydrogenase protein has been isolated fromForsythia intermedia, together with cDNAs encoding secoisolariciresinol dehydrogenase from this species. Accordingly, isolated DNA sequences are provided which code for the expression of secoisolariciresinol dehydrogenase. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a secoisolariciresinol dehydrogenase protein, or to a base sequence sufficiently complementary to at least a portion of a secoisolariciresinol dehydrogenase DNA or RNA to enable hybridization therewith. Thus, systems and methods are provided for the recombinant expression of secoisolariciresinol dehydrogenases that may be used to facilitate the production, isolation and purification of significant quantities of recombinant secoisolariciresinol dehydrogenase for subsequent use, to obtain expression or enhanced expression of secoisolariciresinol dehydrogenase in plants in order to enhance, or otherwise alter, lignan biosynthesis, or may be otherwise employed for the regulation or expression of secoisolariciresinol dehydrogenase.
REFERENCES:
Herbers et al. Plant Mol. Biol. 29 (5), 1027-1038 (1995).
Davin, L.B. et al., “Lignin and Lignan Biochemical Paathways in Plants: Unprecedented Discovery in Phenolic Coupling,”Anais de Academia Brasileira de Ciencias, 67(3):363-378 (1995).
Umezawa et al., “Formation of Lignans (−)-Secoisolariciresinol and (−)-Matairesinol with Forsythia intermedia Cell-free Extracts,”J. Biol. Chem., 266(16):10210-10217 (1991).
Umezawa et al.,J. Chem. Soc., Chem Commun., 1990, 1405-1408.
Umezawa et al.,Biochem. Biophys. Res. Commun., 171(3):1008-1014 (1990).
Moenke, G., “Nicotiana tabacum mRNA for short chain alcohol dehydrogenase,”EMBL 'Online!, (abstract) (1997).
Moenke, G., “Nicotiana tabacum scant gene,”EMBL 'Online!, (abstract) (1998).
Iuchi, S., “Vigna unguiculata mRNA for CRPD12 protein,”EMBL 'Online!, (abstract) (1997).
Davin, L.B. et al., “Purification of secoisolariciresinol dehydrogenase,”Plant Physiology, (abstract) (1997).
Dinkova-Kostova, A. et al., “—Pinoresinol/(+)-lariciresinol reductase from Forsythia Intermedia: Protein Purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase,” Journal of Biological Chemistry, 271(46):29473-29482 (1996).
Davin, L.B. et al., “Lignin and Lignan Biochemical Paathways in Plants: Unprecedented Discovery in Phenolic Coupling,”Anais da Academia Brasileira de Ciencias, 67(3):363-378 (1995).
Umezawa et al., “Formation of Lignans (-)-Secoisolariciresinol and (-)-Matairesinol with Forsythia intermedia Cell-free Extracts,”J. Biol. Chem., 266(16):10210-10217 (1991).
Umezawa et al.,J. Chem. Soc. Chem. Commun., 1990, 1405-1408.
Umezawa et al.,Biochem. Biophys. Res. Commun., 17(3):1008-1014 (1990).
Costa Michael A.
Davin Laurence B.
Lewis Norman G.
Xia Zhi-Qiang
Achutamurthy Ponnathapu
Christensen O'Connor Johnson & Kindness PLLC
Pak Yong D.
Washington State University Research Foundation
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