Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-07-16
1999-01-12
Spector, Lorraine
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 697, 43525233, 530381, 530415, 536 231, 536 235, 536 237, 536 241, C07K 14745, C07K 14245, C12N 1512, C12N 510
Patent
active
058587241
ABSTRACT:
Recombinant rabbit tissue factor is cloned and produced in a bacterial host. This protein, which is relatively insoluble and has several disulfide bonds, requires special modifications in order to express and be purified at commercial levels. By expressing the tissue factor as a fusion protein with a bacterial enzyme, thioredoxin, solubility is increased. Use of a thioredoxin reductase deficient host aids in proper tertiary structure for biological activity.
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Domanico Michael J.
Kroeker Warren
Novy, Jr. Robert E.
Yaeger Keith W.
Kaufman Claire M.
Pel-Freez
Spector Lorraine
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