Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1995-06-05
1998-12-29
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
530324, 530350, 530351, 5303877, 5303873, 530399, C07K 1900, C12N 912
Patent
active
058540440
ABSTRACT:
This invention relates to the production and use of recombinant Pseudomonas-derived toxins modified to increase their toxicity and potency in therapy. More particularly, the invention relates to certain deletions in domain II of the amino acid sequence of Pseudomonas exotoxin the domain which relates to the toxin's natural proteolytic processing.
REFERENCES:
patent: 4892827 (1990-01-01), Pastan et al.
patent: 5082927 (1992-01-01), Pastan et al.
Chaudhary et al., Proc. Natl. Acad. Sci. USA, 84:4538-4542 (1987).
Chaudhary et al., Proc. Natl. Acad. Sci. USA, 85:2939-2943 (1988).
Siegall, C.B. et al., "Functional Analysis of Domains II, Ib, and III of Pseudomonas Exotoxin", Journal of Biological Chemistry, 264:14256-14261 (1989).
Lorberboum-Galski, H. et al., "IL2-PE66.sup.4Glu, a New Chimeric Protein cytotoxic to Human-activated T Lymphocytes", Journal of Biological Chemistry, 265:16311-16317 (1990).
Heimbrook, D.C. et al., "Transforming growth factor .alpha.-Pseudomonas exotoxin fusion protein prolongs survival of nude mice bearing tumor xenografts", Proc. Natl. Acad. Sci. USA, 87:4697-4701 (1990).
Chaudhary et al., Proc. Natl. Acad. Sci. USA, 87:308-312 (1990).
Seetharam, S. et al., "Increased Cytotoxic Activity of Pseudomonas Exotoxin and Two Chimeric Toxins Ending in KDEL", Journal of Biological Chemistry, 266:17376-17381 (1991).
Kreitman, R.J. et al., "Properties of Chimeric Toxins with Two Recognition Domains: Interleukin 6 and Transforming Growth Factor .alpha. at Different Locations in Pseudomonas Exotoxin", Bioconjugate Chemistry, pp. 63-68 (1992).
Kreitman, R.J. et al., "Rational Design of a Chimeric Toxin: An Intramolecular Location for the Insertion of Transforming Growth Factor .alpha. within Pseudomonas Exotoxin as a Targeting Ligand", Bioconjugate Chemistry, pp. 58-62 (1992).
Siegall et al., 1991, Biochem. 30,7154-7159.
Gray et al., 1984 Proc. Nat'l. Acad. Sci. USA 81, 2645-2649.
Ogata et al., 1990 J. Biol. Chem. 265, 20678-20685.
Jinno et al., 1989 J. Biol. Chem. 264, 15953-15959.
Pastan et al., Science 254:1173-1177 (1991).
Theuer et al., J. Biol. Chem. 267:16872-16877 (1992).
Fitzgerald David J.
Pastan Ira H.
National Institutes of Health
Prouty Rebecca E.
LandOfFree
Recombinant pseudomonas exotoxin with increased activity does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant pseudomonas exotoxin with increased activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant pseudomonas exotoxin with increased activity will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1423680