Recombinant PRRSV proteins, diagnostic kits and vaccines contain

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...

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4242011, 4241981, 4242041, 4242781, 4241991, 424815, 4352351, 435 5, 435 693, 435325, 4352523, 435 911, 435239, 530350, 536 2372, A61K 3900, A61K 3912, C12N 1500, C12N 700

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058885137

ABSTRACT:
Recombinant proteins of the causative virus of porcine reproductive and respiratory syndrome (PRRS), corresponding to ORFs 2 to 7 of the PRRSV Spanish isolate (PRRS-Olot), have been produced in baculovirus expression system using Sf9 cell cultures as a permissive host. These recombinant proteins are suitable for the formulation of vaccines capable of efficaciously protecting porcine livestock from PRRS and for the preparation of diagnostic kits adequate for detection of anti-PRRSV antibodies as well as of PRRSV in a pig biological sample. This invention is of interest to Veterinary Medicine.

REFERENCES:
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S. J. McCullough, et al., The New Pig Disease, Porcine Respiration and Reproductive Syndrome, A Report on the Seminar/Workshop Held in Brussels on 29-30 Apr. 1991 and Organized by the European Commission (Directorate-General for Agriculture), 46-52.
von V.F. Ohlinger, et al., Der Seuchenhafte Spatabort beim Schwein--Ein Beitrag zur Atiologie des Porcine Reproductive and Respiratory Syndrome (PRRS), Tierarztl. Umschau 46, 703-708 (1991).
James E. Collins, et al., Swine Infertility and Respiratory Syndrome (Mystery Swine Disease), Proc. Minnesota Conf. for Veterinarians, Sep. 15, 1991-Sep. 17, 1991, 200-205.
G. Wensvoort, et al., Mystery swine disease in the Netherlands: the isolation of Lelystad virus, The Veterinary Quarterly, vol. 13, No. 3, 1991, 121-129.
Gert Wensvoort, et al., Lelystad virus, the cause of porcine epidemic abortion and respiratory syndrome: a review of mystery swine disease research at Lelystad, Veterinary Microbiology, 33(1992), 185-193.
C. Terpstra, et al., Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch's postulates fulfilled, The Veterinary Quarterly, vol. 13, No. 3, 1991, 131-136.
J.M.A. Pol, et al., Pathological, ultrastructural, and immunohistochemical changes caused by Lelystad virus in experimentally induced infections of mystery swine disease (synonym: porcine epidemic abortion and respiratory syndrome (PEARS)), The Veterinary Quarterly, vol. 13, No. 3, 137-143 (Jul. 1991).
D.J. Paton, et al., Isolation of a Lelystad virus-like agent from British pigs and scanning electron microscopy of infected macrophages, Veterinary Microbiology, 33 (1992), 195-201.
Joan Plana, et al., Porcine epidemic abortion and respiratory syndrome (mystery swine disease). Isolation in Spain of the causative agent experimental reproduction of the disease, Veterinary Microbiology 33 (1992) 203-211.

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