Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2006-02-14
2006-02-14
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S325000, C435S320100, C435S023000, C435S069100, C435S219000, C435S252300, C435S254100, C435S254200, C435S254230, C536S023200, C530S350000
Reexamination Certificate
active
06998260
ABSTRACT:
Disclosed is a method of expressing enzymatically-active, recombinant proteolytic tryptase in a eukaryotic host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed hosts, and genetically-engineered eukaryotic host cells containing the expression constructs and which express enzymatically-active proteolytic tryptases. Uses for the proteolytic tryptases so produced are also disclosed. Also disclosed is a method of making active site mutants of proteolytic tryptases in a eukaryotic host cell, expression constructs which drive the production of the mutants in transformed eukaryotic host cells, and genetically-engineered eukaryotic host cells containing the expression constructs and which express the active-site mutated form of proteolytic tryptases.
REFERENCES:
Bork , Genome Research, 10:398-400, 2000.
Broun et al. , Science 282:1315-1317, 1998.
Seffernick et al. , J. Bacteriol. 183(8):2405-2410, 2001.
Van de Loo et al. , Proc. Natl. Acad. Sci. 92:6743-6747, 1995.
Blom, T. and Hellman, L. (1993),Scand. J. Immunol.37:203-208.
Buckholz, R.G. and Gleeson, M.A.G. (1991),Biotechnology9:1067-1072.
Chan, H.; Elrod, K.C.; Numerof, R.P. Sideris, S; and Clark, J.M. (1999),Protein Express.&Purif.15:251-257.
Clark J.M.; Abraham, W.M.; Fishman, C.E.; Foreza, R. Ahmed, A.; Cortes, A.; Warne, R.L.; Moore, W.R; and Tanaka, R.D. (1995),Am. J. Respir. Crit. Care Med.152:2076-2083.
Holgate S.T. and Church, M.K. (1992),Br. Med. Bull UK48:40-50.
Faber K.N.; Harder, W.; Ab, G.; and Veenhuis, M. (1995),Yeast11:1331-1344.
Harvima, I.T.; Schechter N.M.; Harvima, R.J.; and Fräki, J.E. (1988) Human Skin Tryptase: Purification, Partial Characterization and Comparison with Human Lung Tryptase,Biochimimica et Biophysica Acta, 957:71-80.
Huang, C.; Li, L.; Krilis, S.A.; Chanasyk, K.; Tang, Y.; Li, Z.; Hunt, J.E.; and Stevens, R.L. (1999) Human Tryptase α and β/II are Functionally Distrinct Due, in Part, to a Single Amino Acid Difference in One of the Surface Loops that Forms the Substrate-binding Cleft,J. Biol. Chem.274:19670-19676.
Huang, C.; Guillermo, M.; Vagi, A.; Chanasyk, K.; Ferrazzi, M.; Burklow, C.; Qiu, W.; Feyfant, E.; Sali, A.; and Stevens, R.L. , (2000) Formation of Enzymatically Active, Homotypic, and Heterotypic Tetramers of Mouse Mast Cell Tryptases,J. Biol. Chem.275:351-358.
Ide, H.; Itoh, H.; Tomita, M.; Murakumo, Y.; Kobayashi, T.; Maruyama, H.; Osada, Y.; and Nawa, Y. (1995),J. Biochem.118:210-215.
Miller, J.S.; Moxley, G.; and Schwartz, L.B. (1990),J. Clin. Invest.86:864-870.
Niles, A.L.; Maffitt, M.; Haak-Frendscho, M.; Wheeless, C.J.; and Johnson, D.A. (1998), Recombinant Human Mast Cell Tryptase: Stable Expression inPichia pastorisand Purification of Fully Active Enzyme,Biotechnol. Appl. Biochem.28:125-131.
Nilsson and Schwartz (1994), Mast-Cell Heterogeneity: Structure and Mediators, Blackwell Scientific Publications, Boston, pp. 195-208.
Pallaoro, M.; Fejzo, M.S.; Shayesteh, L; Blount, J.L.; and Caughey, G.H. (1999), Characterization of Genes Encoding Known and Novel Human Mast Cell Tryptases on Chromosome 16p13.3,J. Biol. Chem.6:3355-3362.
Sakai K.; Long, S.D.; Dove-Pettit, D.A.; Cabral, G.A.; and Schwartz, L.B. (1996),Protein Express.&Purif.7:67-73.
Sambrook, J.; Fritsch, E. F.; and Maniatis, T. (1989),Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: New York, NY.
Schwartz L.B.; Bradford, T.R.; Littman, B.H.; and Wintroub, B.U. (1985),J. Immun.135:2762-2767.
Schwartz L and Bradford T. (1986) Regulation of tryptase from human lung mast cells by heparin: Stabilization of the active tetramer,J. Biol. Chem.256: 7372-7379.
Schwartz (1995), Structure and Function of Human Mast Cell Tryptase,Biological and Molecular Aspects of Mast Cell and Basophil Differentiation and Function, chapter 14:161-173, Raven Press, Ltd: New York.
Scopes, R. K., (1994), Protein Purification, Principles and Practices, Springer-Verlag: New York.
Smith, T.J.; Houghland, M.W.; and Johnson, D.A. (1984),J. Biol. Chem.259(17):11045-11051.
Sommerhoff, C.P.; Bode, W.; Pereira, P.J.B.; Stubbs, M.T.; Stürzebecher, J.; Piechottka, G.P.; Matschiner, G.; and Bergner, A. (1999), “The Structure of the Human βII-tryptase Tetramer: Fo(u)r Better of Worse,”Proc. Natl. Acad. Sci. USA96:10984-10991.
Vanderslice P.; Ballinger, S.M.; Tam, E.K.; Goldstein, S.M.; Craik, C.S.; and Caughey, G. H. (1990),Biochemistry87:3811-3815.
Wung, J.L. and Gascoigne, R.J. (1996),BioTechniques21:812-816.
Haak-Frendscho Mary
Maffitt Mark
Niles Andrew L.
DeWitt Ross & Stevens S.C.
Leone, Esq. Joseph T.
Promega Corporation
Prouty Rebecca E.
Ramirez Delia M.
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