Recombinant protein production with reduced expression of select

Details Recombinant protein production with reduced expression of select Recombinant protein production with reduced expression of select

1993-01-21

1998-02-10

Chambers, Jasemine C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 914, 435 9141, 435 9142, 4353201, 536 241, C12P 2100, C12N 1563, C12N 1565, C07H 2104

Patent

active

057168032

ABSTRACT:
The present invention relates to expression of recombinant proteins by use of a bacterial host expression vector which expresses a recombinant protein under the control of a first regulatory expression element, and expresses a selectable marker under the control of a second regulatory expression element, which second element is mutated such that expression of the selectable marker is at reduced levels relative to that directed by such an unmutated expression element. Such an expression vector in a suitable bacterial host (a) allows ease of purification of the recombinant protein of interest ("the recombinant protein") since less selectable marker is present to interfere with the purification of the recombinant protein, and (b) increases the amount of recombinant protein that is produced by the bacterial host cell. Furthermore, in an embodiment in which the selectable marker is an antibiotic resistance gene, expression according to the present invention ensures that only the minimal necessary levels of antibiotic will be used for selection during fermentation. The invention provides expression vectors, host-vector expression systems (comprising the expression vector in a bacterial host in which it can be expressed), and methods relating thereto.

REFERENCES:
patent: 4530901 (1985-07-01), Weissmann
patent: 4716112 (1987-12-01), Panayotatos
DeBoer et al., 1982, Promoters: Structure and Function pp. 462-481.
Nakamura & Inouye, 1979, Cell 18:1109-1117.
Sambrook et al. (ed.), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 1.5-1.6, 1989.
Schaller et al., 1975, Proc. Natl. Acad. Sci. U.S.A. 72(2):737-741.
Shine and Dalgarno, 1975, Nature 254:34-38.
Pribnow, 1975, Proc. Natl. Acad. Sci. U.S.A. 72(3):784-788.
Takanami et al., 1976, Nature 260:297-302.
Seeburg et al., 1977, Eur. J. Biochem. 74:107-113.
Uhlin and Nordstrom, 1977, Plasmid 1:1-7.
Rosenberg et al., 1979, Ann. Rev. Genet. 13:319-353.
Steitz, 1980, in Ribsomes: Structure, Function and Genetics, Chambliss et al., (eds.), University Park Press, Baltimore, MD, pp. 479-495.
Panayotatos and Truong, 1981, Nucl. Acids. Res. 9(21):5679-5687.
Wong et al., 1982, Proc. Natl. Acad. Sci. U.S.A. 79:3570-3574.
Kalderon et al., 1982, Nucl. Acids Res. 10(17):5161-5171.
Stormo et al., 1982, Nucl. Acids Res. 10(9):2971-2996.
Matteucci and Heyneker, 1983, Nucl. Acids Res. 11(10):3113-3121.
Oostra et al., 1983, Nature 304:456-459.
Panayotatos et al., 1983, J. Cell. Biochem. Suppl. 7B:109 (Abstr. 765).
Panayotatos, 1984, Nucl. Acids Res. 12(6):2641-2648.
Gold et al., 1984, Proc. Natl. Acad. Sci. U.S.A. 81:7061-7065.
Cowing et al., 1985, Proc. Natl. Acad. Sci. U.S.A. 82:2679-2683.
Buell and Panayotatos, 1986, in Gene to Protein: Steps Dictating the Maximal Level of Gene Expression, Reznikoff and Gold (eds.), Butterworths, Stoneham, MA, pp. 345-363.
Derbyshire et al., 1986, Gene 46:145-152.
Stewart et al., 1986, Plasmid 15:172-181.
Panayotatos, 1987a, in Plasmids, A Practical Approach, Hardy (ed.), IRL Press, Washington, D.C., pp. 163-176.
Panayotatos, 1987b, in Vectors A Survey of Molecular Cloning Vectors and their Uses, Rodriguez and Denhardt (eds.), Butterworths, Boston, MA, pp. 227-235.
Panayotatos, 1988, Gene 74:357-363.
Studnicka, 1988, Biochem. J. 252:825-831.
Kues and Shahl, 1989, Microbiol. Rev. 53(4):491-516.
Leibrock et al., 1989, Nature 341:149-152.
Stockli et al., 1989. Nature 342:920-923.

Affiliated with

Also associated with

Rating

Recombinant protein production with reduced expression of select does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Recombinant protein production with reduced expression of select, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant protein production with reduced expression of select will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2076394