Recombinant protein production in serum-free medium

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 691, 4352402, 4352403, 43524031, 4353201, 530359, 530383, C12P 2106, C12N 500, C07K 300, A61K 3514

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053567986

ABSTRACT:
In a method for effecting an increased expression in serum-free medium of recombinant proteins in a host cell being able to express said protein said host is cultured in a serum-free medium comprising an egg yolk fraction being free of lipoprotein and lipids so as to express said protein. Also the use of an egg yolk fraction being free of lipoprotein and lipids for increasing the expression of recombinant proteins in a host cell being able to express said protein in a serum-free cell growth medium is disclosed.

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Dialog Info. Svce., Abstract of Ito H. et al., Nippon Hinyokika Gakkai Zasshi, vol. 80, No. 12, pp. 1741-1748 (1989).
Dialog Info. Svce., Abstract of Martis et al., In Vitro Cell Dev. Biol. vol. 22, No. 5, pp. 241-246 (1986).
Dialog Info. Svce., Abstract of JP 1083100 (1989).

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