Recombinant protein comprising starch binding domain and use...

Details Recombinant protein comprising starch binding domain and use... Recombinant protein comprising starch binding domain and use...

2009-12-21

2010-12-14

Monshipouri, Maryam (Department: 1656)

Chemistry: molecular biology and microbiology

Vector, per se

C536S023100

Reexamination Certificate

active

07851215

ABSTRACT:
A recombinant protein is prepared comprising a polypeptide of interest and a starch binding domain (SBD). The said SBD is obtainable from glucoamylase of fungi genusRhizopus. The said recombinant protein comprising the said SBD can be purified by contacting with an affinity matrix such as starch, the SBD binds to the affinity matrix to isolate the recombinant protein. The recombinant protein can be purified by separating the association between the SBD and the affinity matrix by acid, alkaline, salt, or sugar. The polypeptide of interest may be an antibody, an antigen, a therapeutic compound, an enzyme, or a protein and may apply in pathogen destruction, vaccine producing, and oral care product manufacturing. The SBD further provides as a tool to screen or identify polysacchrides.

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