Recombinant production of novel polyketides

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435183, 4352523, 435325, C12N 121, C12N 510, C12P 2102

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058437185

ABSTRACT:
Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.

REFERENCES:
patent: 4935340 (1990-06-01), Baltz et al.
Yu et al., J. Bacteriol. 176:2627-2634, 1994.
Bartel et al. (1990), "Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: Clarification of actinorodin gene functions," J. Bacteriol. 172:4816-4826.
Bibb et al. (1989), "Analysis of the nucleotide sequence of the Streptomyces glaucescens tcml genes provides key information about the enzymology of polyketide antibiotic biosynthesis," EMBO J. 8:2727.
Caballero et al. (1991), "Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor," Mol. Gen. Genet. 230:401.
Cortes et al. (1990), "An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea," Nature 348:176.
Donadio et al. (1991), "Modular organization of genes required for complex polyketide biosynthesis," Science 252:675.
Donadio et al. (1992), "Biosynthesis of the erythromycin macrolactone and a rational approach for producing hybrid macrolides," Gene 115:97-103.
Fernandez-Moreno et al. (1991), "The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces," Cell 66:769-780.
Fernandez-Moreno et al. (1992), "Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin," J. Biol. Chem. 267:19278-19290.
Floss et al. (1991), "Genetic engineering of hybrid antibiotics--a progress report," Tetrahedron 47(31):6045-6058.
Fu et al. (1994), "Engineered biosynthesis of novel polyketides: Stereochemical course of two reactions catalyzed by a polyketide synthase," Biochemistry 33(31):9321-9326.
Gordon et al. (1996), "Strategy and tactics in combinatorial organic synthesis. Application to drug discovery," Acc. Chem. Res. 29(3):144-154.
Hallam et al. (1988), "Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor," Gene 74:305-320.
Hershberger et al. (1989), Genetics and Molecular Biology of Industrial Microorganisms (Am. Soc. for Microbiol., Washington, DC) pp. 68-84.
Hopwood et al. (1985), "Product of `hybrid` antibiotics by genetic engineering," Nature 314:642-644.
Hopwood et al. (1989), "Antibiotics: opportunities for genetic manipulation," Phil. Trans. R. Soc. Lond. B324:549-562.
Hutchinson (1994), "Drug synthesis by genetically engineered microorganisms," Bio/Technology 12:375-380.
Katz et al. (1993), "Polyketide synthesis: Prospects for hybrid antibiotics," Ann. Review Microbiol. 47:875-912.
Khosla et al. (1992), "Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein," Molec. Microbiol. 6(21):3237-3249.
Khosla et al. (1993), "Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins," J. Bacteriol. 175:2197-2204.
MacNeil et al. (1992), "Complex organization of the Streptomyces avermitilis genes encoding the avermectin polyketide synthase," Gene 115:119-125.
McAlpine et al. (1987), "New Antibiotics from Genetically Engineered Actinomycetes: I. 2-Norerythromycins, Isolation and Structural Determinations," J. Antibiotics, 40(8):1115-1122.
McDaniel et al. (1993), "Engineered biosynthesis of novel polyketides," Science 262:1546-1550.
Malpartida et al. (1984), "Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes," Nature 309:462.
Malpartida et al. (1986), "Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2)," Mol. Gen. Genet. 205:66-73.
Robinson (1991), "Polyketide synthase complexes: their structure and functions in antibiotic biosynthesis," Phil. Trans. R. Soc Land B. 332:107-114.
Roberts et al. (1992), "6-Deoxyerythronolide B synthase 3 from Saccharopolyspora erythraea: Over-expression in Escherichia coli, purification and characterisation," Biochem. Soc. Trans. 21:325.
Roberts et al. (1993), "Heterologous expression in Escherichia coli of an intact multienzyme component of the erythromycin-producing polyketide synthase," Eur. J. Biochem. 214:305-311.
Rohr (1995), "Combinatorial biosynthesis-an approach in the near future?" Angew. Chem. Int. Ed. Engl. 34(8):881-885.
Sherman et al. (1992), "Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by hetergenous genes from a different polyketide pathway," J. Bacteriol. 174:6184-6190.
Sherman et al. (1989), "Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber," EMBO J. 8:2717-2725.
Strohl et al. (1992), "Significance of anthraquinone formation resulting from the cloning of actinorhodin genes in heterologous streptomycetes," Molecular Microbiology 6(2):147-152.
Strohl et al. (1991), "Expression of polyketide biosynthesis and regulatory genes in heterologous streptomycetes," J. Ind. Microbiol. 7:163-174.
Tsoi et al. (1995), "Combinatorial biosynthesis of unnatural and natural products: the polyketide example," Database Caplus on STN, Chemical Abstract No. 123:169385.
Tuan et al. (1990), "Cloning of Genes Involved in Erythromycin biosynthesis from Saccharopolyspora erthraea using a novel actinomycete-Eschericia coli cosmid," Gene 90:21-29.

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