Recombinant production of novel polyketides

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435 76, 435 914, 435183, 4352402, 43525235, 4353201, C12N 121, C12N 900, C12N 1563, C12P 1934

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056724918

ABSTRACT:
Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.

REFERENCES:
patent: 4935340 (1990-06-01), Baltz et al.
Tuan et al., Gene 90:21-29 (1990).
Roberts et al., Eur. J. Biochem. 214:305-311 (1993).
Roberts et al., Biochem. Soc. Transactions 21:325 (1992).
Hopwood, Phil. Trans. R. Soc. Lond. B 324:549-562 (1989).
Robinson, Phil. Trans. R. Soc. Lond. B 332:107-114 (1991).
Bartel et al., Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: Clarification of actinorhodin gene functions,J. Bacteriol. (1990) 172:4816-4826.
Bibb, M.J. et al., Analysis of the nucleotide sequence of the Streptomyces glaucescens tcml genes provides key information about the enzymology of polyketide antibiotic biosynthesis, EMBO J. (1989) 8:2727.
Caballero, J. et al., Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor, Mol. Gen. Genet. (1991) 230:401.
Cortes, J. et al., An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea, Nature (1990) 348:176.
Donadio, S. et al., Modular organization of genes required for complex polyketide biosynthesis, Science (1991) 252:675.
Fernandez-Moreno, M.A. et al., The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces, Cell (1991) 66:769.
Fernandez-Moreno, M.A. et al., Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin,J. Biol. Chem. (1992) 267:19278.
Hallam, S.E. et al., Gene (1988) 74:305-320.
Hopwood, D.A. et al., Product of `hybrid` antibiotics by genetic engineering,Nature (1985) 314:642-644.
Hutchinson, Drug synthesis by genetically engineered microorganisms, Bio/Technology (1994) 12:375-380.
Khosla, C. et al., Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins, J. Bacteriol. (1993) 175:2197-2204.
MacNeil, D.J. et al., Gene (1992) 115:119-125.
Malpartida, F. and Hopwood, D.A. Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2),Mol. Gen. Genet. (1986) 205:66-73.
Malpartida, F. and Hopwood, D.A. Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genesNature (1987) 325:818-821.
Sherman, D.H. et al., Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by hetergenous genes from a different polyketide pathway, J. Bacteriol. (1992) 174:6184-6190.
Sherman, D.H. et al., Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tu22, EMBO J. (1989) 8:2717-2725.
Fu et al., "Engineered biosynthesis of novel polyketides: Stereochemical course of two reactions catalyzed by a polyketide synthase" Biochemistry (1994) 33(31):9321-9326.
McDaniel et al., "Engineered biosynthesis of novel polyketides" Science (3 Dec. 1993) 262:1546-1550.
Floss et al., "Genetic engineering of hybrid antibiotics--a progress report" Tetrahydron (1991) 47(31):6045-6058.
Donadio et al., "Biosynthesis of the erythromycin macrolactone and a rational approach for producing hybrid macrolides" Gene (1992) 115:97-103.
Hershberger et al., Genetics and Molecular Biology of Industrial Microorganisms (1989) Am. Soc. for Microbiol. (Washington, DC) pp. 68-84.
Katz et al., "Polyketide synthesis: Prospects for hybrid antibiotics" Ann. Review Microbiol. (1993) 47:875-912.
Khosla et al., "Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein", Molec. Microbiol. (1992) 6(21):3237-3249.

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