Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1999-03-05
2000-06-20
Nashed, Nashaat T.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435132, 435147, 435148, 435183, 4352523, 43525233, 43525235, 4353201, 536 231, 536 232, C12P 762, C12P 724, C12N 900
Patent
active
06077696&
ABSTRACT:
Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.
REFERENCES:
patent: 4935340 (1990-06-01), Baltz et al.
patent: 5824513 (1998-10-01), Katz et al.
Bartel, et al., "Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: Clarification of actinorhodin gene functions," J. Bacteriol (1990) 172(9):4816-4826.
Beck, et al., "The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Its gene structure relative to that of other polyketide synthases," Eur J Biochem (1990) 192:487-498.
Bibb, et al., "Analysis of the nucleotide sequence of the Streptomyces glaucescens tcml genes provides key information about the enzymology of polyketide antibiotic biosynthesis," EMBO J (1989) 8(9):2727-2735.
Caballero et al., "Organisation and functions of the actVA region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor, " Mol Gen Genet (1991) 230:401-412.
Cortes et al., "n unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea, " Nature (1990) 348:176-178.
Davis, et al., "Functional mapping of a polyketide synthase from Aspergillus terreus involved in lovastatin synthesis," Abst of the Genetics of Industrial Microorganisms Mtg (1994) P288:192.
Donadio et al., "Modular organization of genes required for complex polyketide biosynthesis," Science (1991) 252:675-679.
Donadio et al., "Biosynthesis of the erythromycin macrolactone and a rational approach for producing hybrid macrolides," Gene (1992) 115:97-103.
Fernandez-Moreno et al., "the act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of Streptomyces, " Cell (1991) 66:769-780.
Fernandez-Moreno et al., "Nucleotide sequence and deduced functions of a set cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin," J Biol Chem (1992) 267:19278-19290.
Floss, "Genetic engineering of hybrid antibiotics--a progress report," Tetrahydron (1991) 47(31):6045-6058.
Fu, "Engineered biosynthesis of novel polyketides: Stereochemical course of two reactions catalyzed by a polyketide synthase," Biochemistry (1994) 33(31):9321-9326.
Hallam, "Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor," Gene (1988) 74:305-320.
Hershberger et al., "Genetics and molecular biology of industrial microorganisms," Am Soc for Microbiol (1989) (Washington D.C.) pp. 68-84.
Hopwood et al., "Antibiotics: opportunities for genetic manipulation," Phil Trans R Soc Lond (1989) B324:549-562.
Hopwood et al., "Product of `hybrid` antibiotics by genetic engineering," Nature (1985) 314 (6012):642-644.
Hutchinson, "Drug synthesis by genetically engineered microorganisms," Ann Review Microbiol (1993) 47:875912.
Katz et al., "Polyketide synthesis: Prospects for hybrid antibiotics," Ann. Review Microbiol (1993) 47:875-912.
Khosla, et al., "Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein," Mole Microbiol (1992) 6(21):3237-3249.
Khosla, et al., "Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins," J Bacteriol (1993) 175:2197-2204.
MacNeil et al., "Complex organization of the Streptomyces avermitilis genes encoding the avermectin polyketide synthase," Gene (1992) 115:119-125.
Malpartida et al., "Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host," Nature (1984) 309:462-464.
Malpartida et al., "Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3 (2)," Mol Gen Genet (1986) 205:66-73.
Malpartida et al., "Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes," Nature (1987) 325(6107):818-821.
McDaniel et al., "Engineered biosynthesis of novel polyketides," Science (1993) 262:1546-1550.
Roberts et al., "6-Deoxyerythronolide B synthase 3 from Saccaropolyspora erythraea: Over-expression in Escherichia coli, purification and characterisation," Biochem Soc Trans (1992) 21:325.
Roberts, et al., "Heterologous expression in Escherichia coli of an intact multienzyme component of the erythromycin-producing polyketide synthase," Eur J Biochem (1993) 214:305-311.
Robinson, "Polyketide synthase complexes: their structure and function in antibiotic biosynthesis," Phil Trans R Soc Land B (1991) 332:107-114.
Rohr, "Combinatorial biosynthesis--an approach in the near future?" Angew Chem Int Ed Engl (1995) 34(8):881-885.
Sherman et al., "Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tu22, " EMBO J(1989) 8:2717-2725.
Sherman et al., "Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by heterogenous genes from a different polyketide pathway," J Bacteriol (1992) 174:6184-6190.
Strohl, et al., "Expression of polyketide biosynthesis and regulatory genes in heterologous streptomycetes," J Ind Microbiol (1991) 7:163-174.
Strohl et al., "Significance of anthraquinone formation resulting from the cloning of actinorhodin genes in heterologous streptomycetes," Molecular Microbiology (1992) 6(2):147-152.
Tsoi, et al., "Combinatorial biosynthesis of unnatural and natural products: the polyketide example," Database Caplus on STN, Chemical Abstract No. 123:169385.
Tuan et al., "Cloning of Genes Involved in Erythromycin biosynthesis from Saccharopolyspora erythrae using a novel actinomycete-Escherichia coli cosmid," Gene (1990) 90:21-29.
Tsoi, et al., "Combinatorial biosynthesis of unnatural and natural products: the polyketide example," Chem. Biol., 2(6):355-362.
Ebert-Khosla Suzanne
Fu Hong
Hopwood David A.
Kao Camilla
Khosla Chaitan
Nashed Nashaat T.
The Johns Innes Institute
The Leland Stanford Junior University
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