Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-04-03
1999-06-15
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 697, 4352523, 4352542, 4353201, 530350, 536 232, 536 234, 536 2432, C12N 1500, C12N 1563, C07K 14195, C07H 2104
Patent
active
059121408
ABSTRACT:
Recombinant nucleic acids which encode aminoacyl-tRNA sythetases of pneumocystis origin or portions of such enzymes, have been isolated. These nucleic acids can be used to make expression constructs and transformed host cells for the production of pneumocystis aminoacyl-tRNA synthetases. They can also be used in the further isolation of nucleic acids related by DNA sequence similarities, which also encode pneumocystis aminoacyl-tRNA synthetases, or portions thereof. A further embodiment of the invention is antisense nucleic acid which can hybridize to the nucleic acid which encodes the aminoacyl-tRNA synthetase of pneumocystis. The invention also relates to enzymes, isolated and/or recombinant pneumocystis aminoacyl-tRNA synthetases. Antibodies which bind to these enzymes can be made and can be used in the purification and study of the enzymes. Tester strains, which are cells engineered to rely on the function of the tRNA synthetase encoded by an introduced cloned gene, can be used to test the effectiveness of drug candidates in the inhibition of the essential tRNA synthetase enzyme encoded by an introduced cloned pneumocystis gene.
REFERENCES:
patent: 4713337 (1987-12-01), Jasin et al.
patent: 4788148 (1988-11-01), Nilsson et al.
patent: 4952501 (1990-08-01), Jasin et al.
patent: 4963487 (1990-10-01), Schimmel et al.
patent: 5370995 (1994-12-01), Hennecke et al.
Webster, T., et al., "Specific Sequence Homology and Three-Dimensional Structure of an Aminoacyl Transfer RNA Synthetase," Science, 226:1315-1317, Dec. 1984.
Edwards, H., et al., "An E. coli Aminoacyl-tRNA Synthetase Can Substitute for Yeast Mitochondrial Enzyme Function In Vivo," Cell, 51:643-649, Nov. 20, 1987.
Englisch, U., et al., "Structure of the Yeast Isoleucyl-tRNA Synthetase Gene (ILS1)," Biol. Chem. Hoppe-Seyler, 368:971-979, Aug. 1987.
Martindale, D.W., et al., "Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)," Current Genetics, 15:99-106 (1989).
Jenal, U., et al., "Isoleucyl-tRNA Synthetase of Methanobacterium thermautotrophicum Marburg," The Journal of Biological Chemistry, 266(16) :10570-10577, Jun. 5, 1991.
Racher, K.I., et al., "Expression and Characterization of a Recombinant Yeast Isoleucyl-tRNA Synthetase," The Journal of Biological Chemistry, 266(26) :17158-17164, Sep. 15, 1991.
Csank, C., et al., Isoleucyl-tRNA Synthetase from the Ciliated Protozoan Tetrahymena thermophila, The Journal of Biological Chemistry, 267(7) :4592-4599, Mar. 5, 1992.
Shiba, K., et al., "Human cytoplasmic isoleucyl-tRNA synthetase: Selective divergence of the anticodon-binding domain and acquisition of a new structural unit," Proc. Natl. Acad. Sci. USA, 91:7435-7439, Aug. 1994.
von der Haar, F. et al, "Target Directed Drug Synthesis: The Aminoacyl-tRNA Synthetases as Possible Targets," Angew. Chem. Int. Ed., 20(3) :217-302 (1981).
Chalker, A.F., et al., "Analysis and Toxic Overexpression in Escherichia coli of a Staphylococcal Gene Encoding Isoleucyl-tRNA Synthetase," Gene, 141:103-108 (1994).
Hughes, J. and Mellows, G., "Interaction of Pseudomonic Acid A with Escherichia coli B Isoleucyl-tRNA Synthetase," Biochem J., 191:209-219 (1980).
Weygand-Durasevic, I., et al., "Yeast Seryl-tRNA Synthetase Expressed in Escherichia coli Recognizes Bacterial Serine-Specific tRNAs in vivo," Eur. J. Biochem., 214:869-877 (1993). Nitroanilino) -Phenyl!-S-(.beta.-Carboxyethyl) -Dithiocarbamic Acid-Ester with Arginyl-tRNA-Synthetase from Dirofilaria immitis," Trop. Med. Parasit., 36:230-232 (1985).
Shepard, A., et al., "RNA Binding Determinant in Some Class I tRNA Synthetases Identified by Alignment-Guided Mutagenesis," Proc. Natl. Acad. Sci. USA, 89:9964-9968 (1992).
Shiba, K. and Shimmel, P., "Functional Assembly of a Randomly Cleaved Protein," Proc. Natl. Acad. Sci. USA, 89:1880-1884 (1992).
Iaccarino, M. and Berg, P., "Isoleucine Auxotrophy as a Consequence of a Mutationally Altered Isoleucyl-Transfer Ribonucleic Acid Synthetase," J. Bacteriol., 105:527-537 (1970).
Meinnel, T., et al., "Aminoacyl-tRNA Synthetases: Occurrence, Structure, and Function. " In tRNA: Structure, Biosynthesis, and Function, Soll, D. and RajBhandary, U., eds. (Washington, DC: American Society for Microbiology), pp. 251-300 (1995).
Jasin, M. and Schimmel, P., "Deletion of an Essential Gene in Escherichia coli by Site-Specific Recombination with Linear DNA Fragments," J. Bacteriol., 159(2) :783-786 (1984).
Edwards, H. and Schimmel, P., "A Bacterial Amber Suppressor in Saccharomyces cerevisiae Is Selectively Recognized by a Bacterial Aminoacyl-tRNA Synthetase," Molecular and Cellular Biology, 10(4) :1633-1642 (1990).
Shiba, K., et al., "Isolation of Higher Eukaryote Aminoacyl-tRNA Synthetase Genes by an Alignment-Guided Cross-Species PCR: Application to Human International tRNA Workshop, Societe Francaise de Biochimie et Biologie Moleculaire, Cap d'Agde, France, May 30-Jun. 4 (1993), Abstract No. 364.
Printout of a computer record of parts of a poster presented at Cap d'Agde, France, May 30-Jun. 4, 1993, 15th International tRNA Workshop, Societe Francaise de Biochimie et Biologie Moleculaire.
Sequence of Human Isoleucyl-tRNA Synthetase Gene, GenBank Name: HSU04953, Accession: U04953, National Center for Biotechnology Information Seq ID: 450850. Entered by Nichols, R.C., et al. First Available: Jan. 13, 1994; Updated: Jan. 26, 1994.
Hughes, J., et al., "Inhibition of Isoleucyl-Transfer Ribonucleic Acid Synthetase in Escherichia coli by Pseudomonic Acid," Biochem. J., 176:305-318 (1978), Great Britain.
Mirande, M. and Waller, J-P., "The Yeast Lysyl-tRNA Synthetase Gene," The Journal of Biological Chemistry, 263(34) :18443-18451 (1988).
Chan, V. L., and Bingham, H. L., "Lysyl-tRNA Synthetase Gene of Campylobacter jejuni," Journal of Bacteriology, 174(3) :695-701 (1992).
Gatti, D. L., and Tzagoloff, A., "Structure and Evolution of a Group of Related Aminoacyl-tRNA Synthetases," J. Mol. Biol., 218:557-568 (1991).
Leveque, F., et al., "Homology of lysS and lysU, the Two Escherichia coli Genes Encoding Distinct Lysyl-tRNA Synthetase Species," Nucleic Acids Research, 18(2) :305-312 (1990).
Kawakami, K., et al., "Chromosomal Location and Structure of the Operon Encoding Peptide-Chain-Release Factor 2 of Escherichia coli," Proc. Natl. Acad. Sci. USA, 85:5620-5624 (1988).
Clark, R. L., and Neidhardt, F. C., "Roles of the Two Lysyl-tRNA Synthetases of Escherichia coli: Analysis of Nucleotide Sequences and Mutant Behavior," Journal of Bacteriology, 172(6) :3237-3243 (1990).
Sequence of Saccharomyces cerevisiae Cytoplasmic Alanyl-tRNA Synthetase Gene, Complete cds, GenBank Accession: U18672. Entered by Ripmaster, T. L. First available Jan. 2, 1995.
Herlihy, W. C., et al., "Mass Spectra of Partial Protein Hydrolysates as a Multiple Phase Check for Long Polypeptides Deduced from DNA Sequences: NH.sub.2 -Terminal Segment of Alanine tRNA Synthetase," Proc. Natl. Acad. Sci. USA, 77(11) :6531-6535 (1980).
Chang, P. K. and Dignam, J. D., "Primary Structure of Alanyl-tRNA Synthetase and the Regulation of Its mRNA Levels in Bombyx mori," The Journal of Biological Chemistry, 265(34) :20898-20906 (1990).
Selbitschka, W., et al., "Characterization of recA Genes and recA Mutants of Rhizobium meliloti and Rhizobium leguminosarum Biovar viciae," Mol. Gen. Genet, 229:86-95 (1991).
Putney, S.D., et al., "Primary Structure of a Large Aminoacyl-tRNA Synthetase," Science, 213:1497-1501 (1981).
Sequence of Arabidopsis thaliana Alanyl-tRNA Synthetase Gene. Mireau, H., et al., EMBL Accession No. Z22673. File created May 7, 1993.
Kim, S., et al., "Diversified Sequences of Peptide Epitope for Same-RNA Recognition," Proc. Natl. Acad. Sci. USA, 90:10046-10050 (1993).
Archibold, E.R., and Williams, L.S., "Regulation of Methionyl-Transfer Ribonucleic Acid Synthetase Formation in Escherichia coli and Salmonella typhimurium," Journal of Bacteriology, 114(3) :1007-1013 (1973).
Tzagoloff, A., et al., "Characterization of MSM1, the Structural Gene for Yeast Mitochondrial Methionyl-tRNA Synthetase," Eur. J. Biochem., 179:365-371 (1989).
Dardel, F., et al., "Molecular Cloning a
Politis-Virk Karen I.
Quinn Cheryl L.
Schimmel Paul R.
Tao Niajun
Whoriskey Susan K.
Cubist Pharmaceuticals, Inc.
Degen Nancy
Larson Thomas G.
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