Recombinant platelet collagen receptor glycoprotein vi and...

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Reexamination Certificate

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C530S350000

Reexamination Certificate

active

07928066

ABSTRACT:
The invention relates to Glycoprotein VI (GPVI), its isolation, purification, and methods for recombinant production. Especially, the invention relates to the use of GPVI, preferably recombinant GPVI, in the treatment of disorders and pathological events correlated directly or indirectly to blood coagulation disorders such as thrombotic and cardiovascular diseases. The extracellular recombinant protein can also be used for establishing screening assays to find potential inhibitors of the membrane bound GPVI in order to inhibit binding of thrombocytes and platelets, respectively, to collagen. Changes in GPIV can be used to monitor platelet age and exposure to thrombiotic and cardiovascular diseases.

REFERENCES:
patent: 9511259 (1995-04-01), None
Ishibashi et al. International Journal of Hematology 62: 107-115, 1995.
Gibbons Jonathan M et al.: “Glycoprotein VI is the collagen receptor in platelets which underlies tyrosine phosphorylation of the Fc receptor gamma-chain.” FEBS Letters, vol. 413 No. 2, 1997, pp. 255-259.
Jandrot-Perrus Martine et al.: “Adhesion and activation of human platelets induced by convulxin involve glycoprotein VI and integrin alpha-2-beta-1.” Journal of Biological Chemistry, vol. 272, No. 43, 1997, pp. 27035-27041.
Gibbons Jonathan M et al.: “The p85 subunit of phosphatidylinositol 3-kinase associated with the Fc receptor gamma-chain and linker for activator of T cells (LAT) in platelets stimulated by collagen and convulxin.” Journal of Biological Chemistry, vol. 273, No. 51, Dec. 18, 1998, pp. 24437-24443.
Clemetson Jeannine et al.: “The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcalphaR and the natural killer receptors.” Journal of Biological Chemistry, vol. 274, No. 41, Oct. 8, 1999, pp. 29019-29024.
Miura Y: “Platelet Glycoprotein VI” EMBL Database, Accession No. AB035073, Jan. 14, 2000.

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