Recombinant Plasmodium vivax merozoite protein-1 p42 vaccine

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Parasitic organism or component thereof or substance...

Reexamination Certificate

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C424S192100, C424S191100, C424S184100, C536S023100, C536S023400, C435S069500, C435S069700

Reexamination Certificate

active

07150875

ABSTRACT:
In this application is the expression and purification of a recombinantPlasmodium vivax(SalI) PvMSP-1 p42. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant PvMSP-1 p42 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

REFERENCES:
patent: WO 9730159 (1997-08-01), None
patent: WO 99/56755 (1999-11-01), None
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Longacre, S. et al., 1994. Plasmodium vivax merozoite surface protein 1 C-terminal recombinant proteins in baculovirus. Molecular and Biochemical Parasitoloty, 64, p. 191-205.
Dutta, S. et al., 2001. Purification, characterization, and immunogenicity of a disulfide cross-linked Plasmodium vivax vaccine candidate antigen, merozoite surface protein 1, expressed inEshcerichia coli.Infection and Immunity 69, p. 5464-5470.

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