Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1988-02-01
1990-08-07
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435320, 935 29, 935 73, C12N 121, C12N 1570
Patent
active
049467905
ABSTRACT:
A recombinant plasmid for the expression of phenylalanine ammonia-lyase (PAL) is constructed by incorporating therein a combined promoter comprising (a) the fusion promoter (the tac promoter) composed of the trp promoter minus 35 region and the lac UV-5 promoter minus 10 region and (b) the P.sub.L promoter of the lambda phage, the tac promoter and the P.sub.L promoter being connected so as to have the same directional property. This recombinant plasmid permits more efficient expression of PAL in Escherichia coli.
REFERENCES:
Gilbert, H. J.; Clark, I. N.; Gibson, R. K.; Stephenson, J. R.; and Tully, M., "Molecular Cloning of the Phenylalanine Ammonia-Lyase Gene from Rhodosporidium Toruloides in Escherichia Coli K-12", Journal of Bacteriology, Jan. 1985, pp. 314-320.
Anson, J. G.; Gilbert, H. J.; Oram, J. D.; and Minton, N. P., "Complete Nucleotide Sequence of the Rhodosporidium Toruloides Gene Coding for Phenylalanine Ammonia-Lyase", Gene 58(1987), 189-199.
Gene, vol. 58, No. 2-3, 1987, pp. 189-199, Anson et al., "Complete Nucleotide Sequence of the Rhodosporidium toruloides gene . . .".
Fukuhara Nobuhiro
Makiguchi Nobuyoshi
Nakajima Yoshiyuki
Sone Satori
Yoshino Setsuo
Knode Marian C.
Mitsui Toatsu Chemicals Inc.
Weimar Elizabeth C.
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