Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2006-10-27
2008-11-18
Campell, Bruce (Department: 1648)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
C424S204100, C424S093100, C435S069300
Reexamination Certificate
active
07452696
ABSTRACT:
Disclosed is a recombinant plasmid for expressing hepatitis B viral antigens in vivo, comprising an adeno-associated virus (AVV) vector and a replication-competent hepatitis B virus genome fragment. Mice hydrodynamically injected with the recombinant plasmid of the present invention show persistent expression of hepatitis B viral antigens for more than 6 months in the hepatocytes, thus a immuno-competent mouse model for persistent expression of hepatitis B antigens and also for human chronic hepatitis B virus infection is established, which can be applied in evaluation and elucidation of mechanism of chronic hepatitis and anti-viral drug discovery research.
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patent: 2005/0197313 (2005-09-01), Roelvink et al.
Arad et al., Liver-Targeted Gene Therapy by SV40-Based Vectors Using the Hydrodynamic Injection Method, 2005, Human Gene Therapy, vol. 16, pp. 361-371.
Priscilla I. Yang, Alana Althage, Josan Chung, Fracis V. Chisari, Hydrodynamic Injection of Viral DNA: A Mouse Model of Acute Hepatitis B Virus Infection, PNAS, Oct. 15, 2002, 13825-13830, vol. 99, No. 21.
F. Liu, Y.K. Song, D. Liu; Hydrodynamics-Based Transfection in Animals by Systemic Administration of Plasmid DNA, Gene Therapy (1999) 6, 1258-1266.
Chen Ding-Shinn
Chen Pei-Jer
Huang Li-Rung
Wu Hui-Lin
Blumel Benjamin P
Campell Bruce
King Anthony
National Taiwan University
WPAT, P.C.
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