Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2007-07-10
2007-07-10
Kemmerer, Elizabeth (Department: 1649)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C435S007500, C435S069100, C435S320100, C435S325000
Reexamination Certificate
active
10400630
ABSTRACT:
This invention is to provide a photoprotein which binds with a ligand specific for a substance to be detected at a binding ratio of 1:1 such that the luminescence activity is not reduced by binding with the ligand, a conjugate comprising the luminescent photoprotein and ligand, and a substance detection method which employs the conjugate as a marker. A calcium-binding photoprotein is produced having cysteine residue introduced within the 4th amino acid residue from the amino-terminus. A conjugate is formed by binding a ligand specific for a substance to be detected to the calcium-binding photoprotein, in a binding ratio of 1:1, via the introduced cysteine residue. The conjugate may be utilized as a marker for a substance to be detected.
REFERENCES:
Lewis and Daunert. Anal Chem. 2001; 73: 3227-3233.
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J. C. Lewis, et al. Site-Specifically Labeled Photoprotein-Thyroxine Conjugates Using Aequorin Mutants Containing Unique Cysteine Residues: Applications For Binding Assays (Part II) Bioconjugate Chem. 11, 2000, pp. 140-145.
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J. C. Lewis, et al. “Bioluminescence and Secondary Structure Properties of Aequorin Mutants Produced for Site-Specific Cunjugation and Immobilization” Bioconjugate Chem. 11, 2000 pp. 65-70.
Midori Nomura, et al. “A C-Terminal Proline is Required for Bioluminescence of the Ca2+-Binding Photoprotein, Aequorin” Federation of European Biochemical Societies, vol. 295, No. 1,2,3, Dec. 1999, pp. 63-66.
Satoshi Inouye, et al. “Overexpression and Purification of the Recombinant Ca2+-Binding Protein, Apoaequorin” J. Biochem. 105, 1989, pp. 473-477.
Satoshi Inouye, et al. “High-Level Expression and Purification of Apoaequorin” Protein and Expression and Purification 2, 1991, pp. 122-126.
Osamu Shimomura, et al. “The Relative Rate of Aequorin Regeneration From Apoaequorin and Coelenterazine Analogues” Biochem. J. 296, 1993, pp. 549-551.
Borgeest Christina
Chisso Corporation
Kemmerer Elizabeth
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
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